采用混合分子印迹聚合物珠同时测定油样中的玉米赤霉烯酮和交链孢酚真菌毒素。
Simultaneous determination of zearalenone and alternariol mycotoxins in oil samples using mixed molecularly imprinted polymer beads.
机构信息
Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Plaza Ciencias, 2, 28040 Madrid, Spain.
Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Plaza Ciencias, 2, 28040 Madrid, Spain.
出版信息
Food Chem. 2023 Jun 30;412:135538. doi: 10.1016/j.foodchem.2023.135538. Epub 2023 Jan 21.
This work reports the optimization of a method using Molecularly Imprinted Polymers (MIPs) for the simultaneous determination of zearalenone and alternariol mycotoxins. The method was optimized using a chemometric approach where in the optimized conditions, the cartridges with a mixture (50:50, w/w) of both MIPs, were loaded with 30 mL of sample, washed with 2 mL of ACN/water (20/80, v/v) and eluted with 2.5 mL of trifluoroacetic acid/MeOH (3/97, v/v). The extracts were analyzed by HPLC coupled to a fluorescence detector (FLD). The optimized method has been applied and validated to the analysis of the mycotoxins in maize, sunflower and olive oils samples with a limit of detection of 5 and 2 µg kg, respectively. Recoveries were in the range of 94 % to 108 % (RSD < 6 %) for zearalenone and 92 % to 113 % (RSD < 5 %) for alternariol. The results were confirmed by HPLC-MS/MS.
本工作报道了一种使用分子印迹聚合物（MIPs）同时测定玉米赤霉烯酮和交链孢酚霉菌毒素的方法优化。该方法采用化学计量学方法进行优化，在优化条件下，将含有两种 MIPs（50:50，w/w）混合物的试剂盒装入 30 mL 样品，用 2 mL ACN/水（20/80，v/v）洗涤，并用 2.5 mL 三氟乙酸/甲醇（3/97，v/v）洗脱。提取物通过 HPLC 与荧光检测器（FLD）进行分析。该优化方法已应用于玉米、向日葵和橄榄油样品中霉菌毒素的分析，检测限分别为 5 和 2 µg kg。玉米赤霉烯酮的回收率在 94%至 108%（RSD < 6%）之间，交链孢酚的回收率在 92%至 113%（RSD < 5%）之间。结果通过 HPLC-MS/MS 进行了验证。
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