Lai Wei, Shi Yanyang, Zhong Jinbiao, Zhou Xinya, Yang Yang, Chen Zhenyu, Zhang Chunsun
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China; Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China.
Guangzhou First People's Hospital Nansha Hospital, Guangzhou, 511457, China.
Talanta. 2023 May 1;256:124287. doi: 10.1016/j.talanta.2023.124287. Epub 2023 Jan 20.
Liver disease causes serious public health problems because of its high prevalence, particularly affecting low- and middle-income countries. Alanine transaminase (ALT) is considered to be one of the most sensitive indicators for diagnosing liver disease. Although many strategies have been reported for ALT detection, few of them have solved the problem of automatic detection. In this work, for the first time, a dry chemistry-based electrochemiluminescence (DC-ECL) device is developed for point-of-care testing (POCT) of ALT, achieving real sample-to-answer detection. The proposed DC-ECL device consists of the following two components: (a) a DC-ECL chip consisting of the outer shell (including the top cap and pedestal) and detection layer (including the baseplate, electrode pad and carrier pad) and (b) an automatic ECL analyzer mainly including the data processing and instrument control unit, imaging detection unit, electrochemical reaction excitation unit, open detection window unit and rechargeable power supply. Under optimized conditions, the device had a wide detection range (0-1000 U/L), the ECL intensity linearly increased with ALT concentration (5-50 U/L) and logarithmic ALT concentration (50-1000 U/L), and the limit of detection was calculated to be 1.702 U/L. In addition, the DC-ECL device had the ability to measure ALT levels in human serum samples and showed acceptable selectivity, stability and repeatability. These results reveal that the DC-ECL device can overcome the disadvantages of traditional methods for ALT detection (such as high cost and requirement of professional technicians) and potentially opens the door to the development of similar POCT analyzers.
肝脏疾病因其高患病率而导致严重的公共卫生问题，尤其影响低收入和中等收入国家。丙氨酸转氨酶（ALT）被认为是诊断肝脏疾病最敏感的指标之一。尽管已经报道了许多用于ALT检测的策略，但其中很少有解决自动检测问题的。在这项工作中，首次开发了一种基于干化学的电化学发光（DC-ECL）设备用于ALT的即时检测（POCT），实现了从实际样本到答案的检测。所提出的DC-ECL设备由以下两个组件组成：（a）一个DC-ECL芯片，由外壳（包括顶盖和基座）和检测层（包括底板、电极垫和载体垫）组成；（b）一个自动ECL分析仪，主要包括数据处理和仪器控制单元、成像检测单元、电化学反应激发单元、开放检测窗口单元和可充电电源。在优化条件下，该设备具有宽检测范围（0-1000 U/L），ECL强度随ALT浓度（5-50 U/L）和对数ALT浓度（50-1000 U/L）线性增加，计算得出的检测限为1.702 U/L。此外，DC-ECL设备有能力测量人血清样本中的ALT水平，并表现出可接受的选择性、稳定性和重复性。这些结果表明，DC-ECL设备可以克服传统ALT检测方法的缺点（如成本高和需要专业技术人员），并可能为类似的POCT分析仪的开发打开大门。
