Laboratory of Chemical Neuroanatomy, Department of Anatomy, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil.
J Chem Neuroanat. 2023 Apr;129:102241. doi: 10.1016/j.jchemneu.2023.102241. Epub 2023 Feb 2.
The amylin and the melanin-concentrating hormone [MCH] are two peptides related to energetic homeostasis. During lactation, it is possible to locate neurons expressing these peptides in the preoptic area of rat dams. In addition, it was demonstrated that the number of MCH neurons in this region is modulated by litter size. Taken together, the aims of this work were (1) to verify the time course of amylin immunoreactivity during lactation; (2) to verify whether litter size modulates the number of amylin-ir neurons (3) to verify whether there is colocalization between the amylin-ir and MCH-ir neurons. Our results show that (1) there is an increase in the number of amylin-ir neurons during lactation, which reaches a peak at postpartum day 19 and drastically reduces after weaning; (2) there is no correlation between litter size and the number of amylin-ir neurons; and (3) there is minimal overlap between amylin-ir and MCH-ir neurons.
胰岛淀粉样肽和黑色素聚集激素 [MCH] 是与能量稳态相关的两种肽。在哺乳期，可以在大鼠母体的视前区找到表达这些肽的神经元。此外，已经证明该区域的 MCH 神经元的数量可通过产仔数进行调节。综上所述，本研究的目的是：(1) 验证胰岛淀粉样肽免疫反应性在哺乳期的时间进程；(2) 验证产仔数是否调节胰岛淀粉样肽-ir 神经元的数量；(3) 验证胰岛淀粉样肽-ir 与 MCH-ir 神经元之间是否存在共定位。我们的结果表明：(1) 哺乳期胰岛淀粉样肽-ir 神经元的数量增加，在产后第 19 天达到峰值，在断奶后急剧减少；(2) 产仔数与胰岛淀粉样肽-ir 神经元的数量之间没有相关性；(3) 胰岛淀粉样肽-ir 和 MCH-ir 神经元之间几乎没有重叠。
