Park Geun Hee, Lee Si Young, Lee Jong-Bin, Chang Beom-Seok, Lee Jae-Kwan, Um Heung-Sik
Department of Periodontology, Gangneung-Wonju National University College of Dentistry, Gangneung 25457, South Korea.
Department of Microbiology and Immunology, Gangneung-Wonju National University, Gangneung 25457, South Korea; Research Institute of Oral Sciences, Gangneung-Wonju National University College of Dentistry, Gangneung 25457, South Korea.
Photodiagnosis Photodyn Ther. 2023 Mar;41:103317. doi: 10.1016/j.pdpdt.2023.103317. Epub 2023 Feb 3.
This study aimed to evaluate the antimicrobial effect of photodynamic therapy (PDT) against Staphylococcus aureus biofilm on a titanium surface and to compare the differences in the effect of PDT using toluidine blue O (TBO) and methylene blue (MB) as a photosensitizer.
The bacterial strain S. aureus ATCC 25,923 was used. Sandblasted and acid-etched (SLA) disks were divided into the following six groups: phosphate buffer saline (PBS), TBO, MB, PBS with laser (PBS + L), TBO with laser (TBO + L), and MB with laser (MB + L). The laser group samples were irradiated by a cold diode laser for 60 s. After treatment, the number of surviving bacteria was calculated by counting the colony-forming units (CFUs) and confocal laser scanning microscopy (CLSM) was applied to observe the bacteria on the disk surface.
The TBO + L and MB + L groups showed significantly lower CFU/ml than the other groups (p < 0.01). The TBO + L group showed significantly lower CFU/ml than the MB + L group (p = 0.032). There was no significant difference between the PBS, TBO, MB, and PBS + L groups. Within the limitations of this in vitro study, PDT with TBO and MB can effectively reduce S. aureus biofilm on SLA titanium surfaces. TBO is more effective than MB as a photosensitizer. PDT with TBO may be applied to the treatment of peri‑implant disease in the future.
本研究旨在评估光动力疗法（PDT）对钛表面金黄色葡萄球菌生物膜的抗菌作用，并比较使用甲苯胺蓝O（TBO）和亚甲蓝（MB）作为光敏剂的PDT效果差异。
使用金黄色葡萄球菌ATCC 25923菌株。将喷砂和酸蚀（SLA）盘分为以下六组：磷酸盐缓冲盐水（PBS）、TBO、MB、激光照射的PBS（PBS + L）、激光照射的TBO（TBO + L）和激光照射的MB（MB + L）。激光组样本用冷二极管激光照射60秒。处理后，通过计数菌落形成单位（CFU）计算存活细菌数量，并应用共聚焦激光扫描显微镜（CLSM）观察盘表面的细菌。
TBO + L组和MB + L组的CFU/ml显著低于其他组（p < 0.01）。TBO + L组的CFU/ml显著低于MB + L组（p = 0.032）。PBS、TBO、MB和PBS + L组之间无显著差异。在本体外研究的局限性内，TBO和MB的PDT可有效减少SLA钛表面的金黄色葡萄球菌生物膜。TBO作为光敏剂比MB更有效。TBO的PDT未来可能应用于种植体周围疾病的治疗。
