Department of Periodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Laser Research Center of Dentistry, Tehran University of Medical Sciences, Tehran, Iran; Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, Italy.
Photodiagnosis Photodyn Ther. 2019 Sep;27:462-466. doi: 10.1016/j.pdpdt.2019.07.012. Epub 2019 Jul 27.
Antimicrobial Photodynamic therapy (aPDT) is a novel modality suggested for treatment of peri-implantitis. This study aimed to assess the effect of aPDT with toluidine blue (TBO) and indocyanine green (ICG) and 635 nm and 808 nm diode laser on Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) biofilm formed on Laser-Lok titanium discs.
Eighty sterile Laser-Lok titanium discs were inoculated with A. actinomycetemcomitans to form biofilm and were randomly divided into 8 groups (n = 10) of control, chlorhexidine (CHX), TBO, ICG, 635 nm diode laser with 220 mW power, 808 nm diode laser with 250 mW power, 100 μg/mL TBO+635 nm diode laser and ICG+808 nm diode laser. Number of colony forming units (CFUs) on the surface of each disc was counted after the intervention. Data were analyzed using the Kruskal-Wallis test.
Significant differences were noted in colony count among the eight groups after the intervention (P = 0.001). Pairwise comparisons with adjusted P value test showed that aPDT with TBO+635 nm laser and ICG+808 nm laser caused significant reduction of bacterial biofilm compared to the control group (P = 0.0001). TBO alone caused significant reduction of biofilm compared to the control group (P = 0.004). No other significant differences were noted (P > 0.05).
Within the limitations of this study, the results showed that aPDT is a potential modality for decontamination of implant surface and reduction of A. actinomycetemcomitans biofilm in vitro. In this study, aPDT with TBO+635 nm diode laser and ICG+808 nm diode laser decreased the bacterial load on titanium discs.
抗菌光动力疗法（aPDT）是一种用于治疗种植体周围炎的新方法。本研究旨在评估使用甲苯胺蓝（TBO）和吲哚菁绿（ICG）以及 635nm 和 808nm 二极管激光的 aPDT 对在 Laser-Lok 钛盘上形成的伴放线放线杆菌（A. actinomycetemcomitans）生物膜的影响。
将 80 个无菌 Laser-Lok 钛盘接种伴放线放线杆菌以形成生物膜，并将其随机分为 8 组（n=10），分别为对照组、洗必泰（CHX）、TBO、ICG、功率为 220mW 的 635nm 二极管激光、功率为 250mW 的 808nm 二极管激光、100μg/mL TBO+635nm 二极管激光和 ICG+808nm 二极管激光。干预后，计算每个盘表面的菌落形成单位（CFU）数量。使用 Kruskal-Wallis 检验分析数据。
干预后，8 组的菌落计数存在显著差异（P=0.001）。经调整 P 值检验的两两比较显示，与对照组相比，TBO+635nm 激光和 ICG+808nm 激光的 aPDT 显著减少了细菌生物膜（P=0.0001）。TBO 单独处理与对照组相比也显著减少了生物膜（P=0.004）。未观察到其他显著差异（P>0.05）。
在本研究的限制范围内，结果表明 aPDT 是一种潜在的方法，可以对种植体表面进行消毒，并减少体外伴放线放线杆菌生物膜。在这项研究中，TBO+635nm 二极管激光和 ICG+808nm 二极管激光的 aPDT 降低了钛盘上的细菌负荷。
