Medical College, Guangxi University, Nanning, Guangxi 530004, China; Key Laboratory of Biomedical Information Engineering of the Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China.
Medical College, Guangxi University, Nanning, Guangxi 530004, China.
Toxicology. 2023 Mar 1;486:153450. doi: 10.1016/j.tox.2023.153450. Epub 2023 Feb 3.
Cobalt is a kind of heavy metal which is widely used in petrochemical and biomedical industries. Animal studies have reported that cobalt would exert systemic toxicity, but its effects on the ovarian function in mammals, especially for oocyte quality remains unknown. In the present study, we report that cobalt chloride treatment affects ovary coefficient and follicular growth. Oocytes in cobalt chloride exposed mice exhibited a decreased development potential, with the evidence of decreased occurrence rate of germ vesicle breakdown and polar body extrusion. Besides, cobalt chloride disorganized meiotic spindle formation and movement, mechanically associated with affecting TACC3 and Ac-a-tubulin levels, and disturbing actin reorganization. In addition, cobalt chloride exposure result in mitochondrial cristae structures disappear, cluster distribution and potential depolarization. Altogether, these findings suggest that cobalt chloride impairs the ovarian follicle growth and affects oocyte development by disrupted spindle assembly and mitochondrial function.
钴是一种重金属，广泛应用于石化和生物医学工业。动物研究报告称，钴会产生全身毒性，但它对哺乳动物卵巢功能的影响，特别是对卵母细胞质量的影响尚不清楚。在本研究中，我们报告说氯化钴处理会影响卵巢系数和卵泡生长。在氯化钴暴露的小鼠中，卵母细胞的发育潜能下降，表现为生发泡破裂和极体排出的发生率降低。此外，氯化钴打乱了减数分裂纺锤体的形成和运动，这与 TACC3 和 Ac-a-微管蛋白水平的变化以及肌动蛋白重组有关。此外，钴暴露导致线粒体嵴结构消失、聚集分布和潜在去极化。总之，这些发现表明，氯化钴通过破坏纺锤体组装和线粒体功能来损害卵泡生长和卵母细胞发育。
