Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, Taiwan.
Department of Surgery, National Taiwan University Hospital and College of Medicine, 7 Chung-Shan S Rd, Taipei 100, Taiwan.
Int J Biol Macromol. 2023 Apr 1;233:123537. doi: 10.1016/j.ijbiomac.2023.123537. Epub 2023 Feb 3.
Silk fibroin (SF) has good biocompatibility, degradability and mechanical properties. In this study, SF-based microneedle (MN) patches were fabricated as stromal cell-derived factor-1 (SDF-1) carriers that may be used for adipose stem cell (ASC) recruitment. Therefore, SF was chosen as the main MN material to achieve sustained drug release. In addition, the variations in SF-based MN crystallinity after water annealing treatment were also determined. The results indicated that SF-based MN patches were successfully fabricated with a 3M™ commercial template and Polydimethylsiloxane mold. Through optical coherence tomography, it was found that all of the SF-based MN patches prepared in this study had sufficient strength to penetrate the skin to a depth of approximately 400 μm. Sustained release of the model drug-dextran from the SF-based MNs was demonstrated. Although SF-based MNs release SDF-1 in a sustained manner, the quantity released can be regulated and improved. Subsequently, dual-layer SDF-1-loaded MNs fabricated with a gelatin tip and SF body were prepared to enhance SDF-1 release for ASC recruitment. SF-based MNs can show good penetration ability and provide good sustained release while dual-layer MNs can regulate the amount of drug released, which could present an alternative for stem cell therapy.
丝素蛋白（SF）具有良好的生物相容性、可降解性和机械性能。在这项研究中，SF 基微针（MN）贴片被制成基质细胞衍生因子-1（SDF-1）载体，可用于招募脂肪干细胞（ASC）。因此，SF 被选为主要的 MN 材料，以实现药物的持续释放。此外，还确定了水退火处理后 SF 基 MN 结晶度的变化。结果表明，使用 3M™ 商业模板和聚二甲基硅氧烷模具成功制备了 SF 基 MN 贴片。通过光学相干断层扫描发现，本研究中制备的所有 SF 基 MN 贴片都具有足够的强度，能够穿透皮肤至大约 400μm 的深度。从 SF 基 MN 中证明了模型药物葡聚糖的持续释放。尽管 SF 基 MN 以持续的方式释放 SDF-1，但可以调节和改善释放的数量。随后，制备了带有明胶尖端和 SF 体的双层负载 SDF-1 的 MN，以增强 SDF-1 释放以招募 ASC。SF 基 MN 可以显示出良好的穿透能力和良好的持续释放，而双层 MN 可以调节药物释放的量，这为干细胞治疗提供了一种替代方案。
