Department of Food Science and Technology, Faculty of Agricultural Engineering, Sari Agricultural Sciences and Natural Resources University, Km 9 Farah Abad Road, Sari, Iran.
Department of Food Science and Technology, Faculty of Agricultural Engineering, Sari Agricultural Sciences and Natural Resources University, Km 9 Farah Abad Road, Sari, Iran.
Int J Biol Macromol. 2023 Apr 1;233:123557. doi: 10.1016/j.ijbiomac.2023.123557. Epub 2023 Feb 3.
Hyssopus officinalis L (Hyssop) is a good source of phenolic compounds. However, conventional methods for extraction of these compounds typically take a long time and have relatively low recovery rates. This study focused on cold atmospheric plasma (CAP) pretreatment and investigated its effects on the ultrasound-assisted extraction (UAE) of phenolic compounds from hyssop. Hyssop was treated at dielectric barrier discharge plasma with air and nitrogen gases for 5, 10, and 15 min. Optical emission spectroscopy was used to evaluate present active species in the plasma. The water contact angle changes, cell wall polysaccharides distribution, and structural variations of the treated samples were determined after treatment. Antioxidant activity and total phenolic contents (TPC) of the extracts were also evaluated. The results showed that CAP treatment reduced the contact angle making surface more hydrophilic. Compared with hyssop, overall no significant changes in the basic structure of all treated samples or the formation of new functional groups were recognized. In addition, CAP pretreatment before UAE increased the antioxidant activity of extracts according to the FRAP assay than the un-pretreated sample and conventional solvent extraction method. Also, TPC increased in samples treated with nitrogen plasma.
穗甘松香（穗甘松）是酚类化合物的良好来源。然而，这些化合物的常规提取方法通常需要很长时间，并且回收率相对较低。本研究重点研究了冷等离子体（CAP）预处理及其对超声辅助提取（UAE）穗甘松中酚类化合物的影响。用空气和氮气对穗甘松进行介质阻挡放电等离子体处理 5、10 和 15 分钟。使用光发射光谱法评估等离子体中存在的活性物质。处理后测定水接触角变化、细胞壁多糖分布和处理样品的结构变化。还评价了提取物的抗氧化活性和总酚含量（TPC）。结果表明，CAP 处理降低了接触角，使表面更亲水。与穗甘松相比，所有处理样品的基本结构或新官能团的形成均未发生明显变化。此外，与未经预处理的样品和常规溶剂萃取法相比，UAE 前的 CAP 预处理根据 FRAP 测定法增加了提取物的抗氧化活性。而且，氮等离子体处理的样品中 TPC 增加。
