Research Unit LR21ES04, Environmental and Clean Processes Chemistry Faculty of Sciences of Monastir, Monastir University, Monastir, Tunisia.
Department of Chemistry, College of Medicine, AL-Muthanna University, Samawah, Iraq.
Biomed Chromatogr. 2023 May;37(5):e5596. doi: 10.1002/bmc.5596. Epub 2023 Feb 16.
This study was designated to investigate the chemical composition, the antifungal activity and antibiofilm properties of Glycyrrhiza foetida (Desf.) growing in Tunisia and recognized for its pharmacological and therapeutic effects. The chemical analysis of essential oil samples prepared via hydrodistillation of the aerial parts was performed by gas chromatography-mass spectrometry (GC-MS). Moreover, the antifungal activity of G. foetida essential oil was developed against three dermatophyte strains, two molds and Candida spp. yeasts using the broth microdilution assay. According to the percentages, the main constituents are δ-cadinene (13.9%), (E)-caryophyllene (13.2%) and γ-cadinene (8.3%). The efficiency of the essential oil in inhibiting Candida albicans biofilms formation was also evaluated in terms of inhibitory percentages. The results showed that C. albicans and Microsporum canis were the most sensitive to G. foetida essential oil with a complete inhibition at 0.4 and 0.2 mg ml , respectively. Candida albicans biofilm development was reduced by 80% by the volatile oil at a concentration of 0.8 mg ml . The essential oil of G. foetida has a promising role in the control of fungal agents with medical interest and in inhibition of Candida biofilm development.
本研究旨在探讨生长在突尼斯的甘草（Desf.）的化学成分、抗真菌活性和抗生物膜特性，因其具有药理学和治疗作用而备受认可。通过水蒸气蒸馏法从地上部分制备的精油样品的化学分析通过气相色谱-质谱联用（GC-MS）进行。此外，使用肉汤微量稀释法研究了甘草精油对三种皮肤癣菌、两种霉菌和念珠菌属酵母的抗真菌活性。根据百分比，主要成分是δ-杜松烯（13.9%）、（E）-石竹烯（13.2%）和γ-杜松烯（8.3%）。还评估了精油对抑制白色念珠菌生物膜形成的效率。结果表明，白色念珠菌和小孢子菌对甘草精油最敏感，在 0.4 和 0.2 mg/ml 时完全抑制，分别。在 0.8 mg/ml 的浓度下，挥发性油可将白色念珠菌生物膜的发展减少 80%。甘草精油在控制具有医学意义的真菌剂和抑制白色念珠菌生物膜形成方面具有广阔的应用前景。
