Department of Medical Parasitology, Faculty of Medicine, Mansoura University, 2 El Gomhouria Street, Mansoura 35516, Egypt.
Department of Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
J Helminthol. 2023 Feb 6;97:e16. doi: 10.1017/S0022149X22000839.
Biochanin A (BCA) is a multifunctional natural compound that possesses anti-infective, anti-inflammatory, anti-oxidative and hepatoprotective effects. The aim of the study was to assess the therapeutic efficacy of BCA on -infected mice. Fifty mice were divided into six different groups as non-infected, non-infected BCA-treated, infected untreated, early infected BCA-treated (seven days post-infection (dpi)), late infected BCA-treated 60 dpi and infected praziquantel (PZQ)-treated groups. Parasitological, histopathological examination and immunohistochemical staining of transforming growth factor (TGF)-β, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) were investigated in liver sections. Cytochrome P450 (CYP450) gene expression of was evaluated by quantitative real-time polymerase chain reaction RT-qPCR). A single dose of BCA significantly reduced worm burden in early (82.14%) and late infection (77.74%), mean tissue egg load in early (7.27 ± 0.495) and late BCA administration (7.63 ± 0.435) and decreased granuloma size. CYP450 mRNA expression was significantly reduced in early BCA treatment as compared to late treatment which emphasizes that early administration of BCA had more pronounced effects on worms than late administration. Both early and late BCA administration led to significant reduction in inflammatory cytokines as TGF and iNOS. Although the reduction of TGF and iNOS in BCA-treated mice was superior to PZQ, no statistically significant differences were noted. However, a significant downregulation of COX2 was noted in hepatocytes as compared to both infected control and PZQ-treated mice. BCA has schistosomicidal, anti-inflammatory, antioxidant and anti-fibrotic effects and could be regarded as a potential drug in schistosomiasis treatment.
染料木黄酮（BCA）是一种多功能天然化合物，具有抗感染、抗炎、抗氧化和保肝作用。本研究旨在评估 BCA 对感染小鼠的治疗效果。将 50 只小鼠分为 6 个不同组，分别为非感染组、非感染 BCA 处理组、感染未处理组、感染早期 BCA 处理组（感染后 7 天（dpi））、感染晚期 BCA 处理组（60 dpi）和感染吡喹酮（PZQ）处理组。对肝组织进行寄生虫学、组织病理学检查和转化生长因子（TGF）-β、诱导型一氧化氮合酶（iNOS）和环氧化酶（COX-2）的免疫组织化学染色。通过实时定量聚合酶链反应（RT-qPCR）评估 CYP450 基因的表达。单次 BCA 给药可显著降低早期（82.14%）和晚期（77.74%）感染的虫荷，早期（7.27±0.495）和晚期 BCA 给药（7.63±0.435）的组织卵负荷，减少肉芽肿大小。与晚期治疗相比，早期 BCA 治疗可显著降低 CYP450 mRNA 的表达，这表明早期 BCA 给药对蠕虫的作用比晚期给药更为明显。早期和晚期 BCA 给药均可显著降低炎症细胞因子如 TGF 和 iNOS。虽然 BCA 治疗组 TGF 和 iNOS 的减少优于 PZQ，但无统计学差异。然而，与感染对照组和 PZQ 治疗组相比，BCA 治疗组肝细胞中的 COX2 显著下调。BCA 具有杀血吸虫、抗炎、抗氧化和抗纤维化作用，可作为血吸虫病治疗的潜在药物。
