用于纳米颗粒功能化的聚乙二醇共轭物的核磁共振表征
NMR Characterization of Polyethylene Glycol Conjugates for Nanoparticle Functionalization.
作者信息
Pasek-Allen Jacqueline L, Wilharm Randall K, Bischof John C, Pierre Valérie C
机构信息
Department of Biomedical Engineering, University of Minnesota, Twin-Cities, Minneapolis, Minnesota55455, United States.
Department of Chemistry, University of Minnesota, Twin-Cities, Minneapolis, Minnesota55455, United States.
出版信息
ACS Omega. 2023 Jan 18;8(4):4331-4336. doi: 10.1021/acsomega.2c07669. eCollection 2023 Jan 31.
The molecular weight, purity, and functionalization of polyethylene glycols are often characterized by H NMR spectroscopy. Oft-forgotten, the typical H NMR pulse sequence is not C decoupled. Hence, for large polymers, the C coupled H peaks arising from the repeating units have integrations comparable to that of the H of the terminal groups. Ignoring this coupling leads to erroneous assignments. Once correctly assigned, these C coupled H peaks can be used to determine both the molecular weight of the polymer and the efficacy of conjugation of a terminal moiety more accurately than the uncoupled H of the repeating unit.
聚乙二醇的分子量、纯度和功能化通常通过核磁共振氢谱（H NMR）进行表征。常被遗忘的是，典型的H NMR脉冲序列并非碳去耦的。因此，对于大分子聚合物，由重复单元产生的碳耦合氢峰的积分与端基氢的积分相当。忽略这种耦合会导致错误的归属。一旦正确归属，这些碳耦合氢峰可用于比重复单元的未耦合氢更准确地确定聚合物的分子量和末端部分的共轭效率。
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