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基于高光谱和荧光光谱成像的猕猴桃可溶性固形物含量无损检测

Non-destructive detection of kiwifruit soluble solid content based on hyperspectral and fluorescence spectral imaging.

作者信息

Xu Lijia, Chen Yanjun, Wang Xiaohui, Chen Heng, Tang Zuoliang, Shi Xiaoshi, Chen Xinyuan, Wang Yuchao, Kang Zhilang, Zou Zhiyong, Huang Peng, He Yong, Yang Ning, Zhao Yongpeng

机构信息

College of mechanical and electrical engineering, Sichuan Agriculture University, Ya'an, China.

College of Engineering, Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, China.

出版信息

Front Plant Sci. 2023 Jan 18;13:1075929. doi: 10.3389/fpls.2022.1075929. eCollection 2022.

Abstract

The soluble solid content (SSC) is one of the important parameters depicting the quality, maturity and taste of fruits. This study explored hyperspectral imaging (HSI) and fluorescence spectral imaging (FSI) techniques, as well as suitable chemometric techniques to predict the SSC in kiwifruit. 90 kiwifruit samples were divided into 70 calibration sets and 20 prediction sets. The hyperspectral images of samples in the spectral range of 387 nm1034 nm and the fluorescence spectral images in the spectral range of 400 nm1000 nm were collected, and their regions of interest were extracted. Six spectral pre-processing techniques were used to pre-process the two spectral data, and the best pre-processing method was selected after comparing it with the predicted results. Then, five primary and three secondary feature extraction algorithms were used to extract feature variables from the pre-processed spectral data. Subsequently, three regression prediction models, i.e., the extreme learning machines (ELM), the partial least squares regression (PLSR) and the particle swarm optimization - least square support vector machine (PSO-LSSVM), were established. The prediction results were analyzed and compared further. MASS-Boss-ELM, based on fluorescence spectral imaging technique, exhibited the best prediction performance for the kiwifruit SSC, with the , and RPD of 0.8894, 0.9429 and 2.88, respectively. MASS-Boss-PLSR based on the hyperspectral imaging technique showed a slightly lower prediction performance, with the , , and RPD of 0.8717, 0.8747, and 2.89, respectively. The outcome presents that the two spectral imaging techniques are suitable for the non-destructive prediction of fruit quality. Among them, the FSI technology illustrates better prediction, providing technical support for the non-destructive detection of intrinsic fruit quality.

摘要

可溶性固形物含量(SSC)是描述水果品质、成熟度和口感的重要参数之一。本研究探索了高光谱成像(HSI)和荧光光谱成像(FSI)技术,以及用于预测猕猴桃中SSC的合适化学计量技术。将90个猕猴桃样本分为70个校正集和20个预测集。采集了样本在387nm1034nm光谱范围内的高光谱图像以及在400nm1000nm光谱范围内的荧光光谱图像,并提取了它们的感兴趣区域。使用六种光谱预处理技术对这两种光谱数据进行预处理,并将其与预测结果进行比较后选择最佳预处理方法。然后,使用五种主特征提取算法和三种次特征提取算法从预处理后的光谱数据中提取特征变量。随后,建立了三种回归预测模型,即极限学习机(ELM)、偏最小二乘回归(PLSR)和粒子群优化-最小二乘支持向量机(PSO-LSSVM)。进一步对预测结果进行分析和比较。基于荧光光谱成像技术的MASS-Boss-ELM对猕猴桃SSC的预测性能最佳,其决定系数、均方根误差和相对分析误差分别为0.8894、0.9429和2.88。基于高光谱成像技术的MASS-Boss-PLSR的预测性能略低,其决定系数、均方根误差和相对分析误差分别为0.8717、0.8747和2.89。结果表明,这两种光谱成像技术适用于水果品质的无损预测。其中,FSI技术的预测效果更好,为水果内在品质的无损检测提供了技术支持。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/695a/9889828/30e7a84beded/fpls-13-1075929-g001.jpg

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