一种差分脉冲极谱血清蛋白测定法。

A differential pulse polarographic serum protein assay.

作者信息

Hertl W

机构信息

Research and Development Division, Corning, New York 14831.

出版信息

Anal Biochem. 1987 Jul;164(1):1-4. doi: 10.1016/0003-2697(87)90358-7.

DOI:10.1016/0003-2697(87)90358-7

PMID:3674362

Abstract

Human serum and albumin show a differential pulse polarographic band near -0.6 V. For sufficiently dilute solutions (less than 7 micrograms/ml) at pH 7.0, the polarographic response is linearly related to the solution concentration. At pH 12.6 a near-linear response to 70 micrograms/ml is obtained, but sample equilibration time is slow. The polarographic assay for total serum protein using bovine serum albumin (BSA) to generate a standard curve gave a correlation coefficient of 0.89. The cellular constituents of whole blood do not interfere. The correlation with BSA does not hold for protease-digested serum or albumin.

摘要

人血清和白蛋白在-0.6V附近呈现微分脉冲极谱带。对于pH 7.0时足够稀的溶液（小于7微克/毫升），极谱响应与溶液浓度呈线性相关。在pH 12.6时，对于高达70微克/毫升可获得近似线性响应，但样品平衡时间较慢。使用牛血清白蛋白（BSA）生成标准曲线对总血清蛋白进行极谱测定，相关系数为0.89。全血的细胞成分不产生干扰。对于经蛋白酶消化的血清或白蛋白，与BSA的相关性不成立。