Berkowitz S A
J. T. Baker Chemical Company, Phillipsburg, New Jersey 08865.
Anal Biochem. 1987 Jul;164(1):254-60. doi: 10.1016/0003-2697(87)90394-0.
Rapid preparative scale purification of calmodulin from crude bovine brain extract is achieved in a single chromatographic run by physically coupling two different liquid chromatography columns which employ different separation mechanisms. In this case columns packed with newly commercialized 40-microns silica-based hydrophobic interaction and 5-microns micron silica-based weak anion-exchange chromatography media were used. The only sample preparation required for conducting this purification procedure is the addition of salt to the crude brain supernatant to promote the initial binding of calmodulin to the hydrophobic interaction chromatography media. Chromatography carried out on such linear arrangements of columns has been referred to as linear multidimensional liquid chromatography.
通过物理连接两根采用不同分离机制的不同液相色谱柱，在单次色谱运行中即可从粗制牛脑提取物中快速进行制备规模的钙调蛋白纯化。在这种情况下，使用了填充有新商业化的40微米硅胶基疏水相互作用和5微米硅胶基弱阴离子交换色谱介质的色谱柱。进行此纯化程序所需的唯一样品制备步骤是向粗脑上清液中添加盐，以促进钙调蛋白与疏水相互作用色谱介质的初始结合。在这种柱的线性排列上进行的色谱被称为线性多维液相色谱。
