脂蛋白脂肪酶的酶联免疫测定法。
An enzyme-linked immunoassay for lipoprotein lipase.
作者信息
Goers J W, Pedersen M E, Kern P A, Ong J, Schotz M C
机构信息
Department of Chemistry, California Polytechnic State University, San Luis Obispo 93407.
出版信息
Anal Biochem. 1987 Oct;166(1):27-35. doi: 10.1016/0003-2697(87)90541-0.
Polyclonal antibodies against bovine milk lipoprotein lipase (LPL) were used to generate an enzyme-linked immunosorbent assay (ELISA) for rat LPL. The antibodies to LPL were affinity purified on bovine LPL columns and were shown to be specific for LPL by immunoprecipitation and enzyme inhibition. The solid-phase ELISA was sensitive from 1.0 to 20 ng/ml of LPL and paralleled enzyme activity. Denatured rat LPL showed the same LPL mass as undenatured samples, allowing LPL mass to be quantitated effectively in a variety of rat tissue extracts.
利用抗牛乳脂蛋白脂肪酶（LPL）的多克隆抗体建立了一种针对大鼠LPL的酶联免疫吸附测定法（ELISA）。LPL抗体在牛LPL柱上进行亲和纯化，并通过免疫沉淀和酶抑制证明对LPL具有特异性。固相ELISA对LPL的检测灵敏度为1.0至20 ng/ml，且与酶活性呈平行关系。变性大鼠LPL显示出与未变性样品相同的LPL质量，从而能够在多种大鼠组织提取物中有效定量LPL质量。
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