3D 打印介孔生物活性玻璃、生物玻璃 45S5 和 β-TCP 支架用于再生医学：体外比较研究。
3D printed mesoporous bioactive glass, bioglass 45S5, and β-TCP scaffolds for regenerative medicine: A comparative in vitro study.
机构信息
Biomaterials Division, New York University College of Dentistry, New York, NY, USA.
Department of Molecular Pathobiology, New York University College of Dentistry, New York, NY, USA.
出版信息
Biomed Mater Eng. 2023;34(5):439-458. doi: 10.3233/BME-222524.
BACKGROUND
While autografts to date remain the "gold standard" for bone void fillers, synthetic bone grafts have garnered attention due to their favorable advantages such as ability to be tailored in terms of their physical and chemical properties. Bioactive glass (BG), an inorganic material, has the capacity to form a strong bond with bone by forming a bone-like apatite surface, enhancing osteogenesis. Coupled with additive manufacturing (3D printing) it is possible to maximize bone regenerative properties of the BG.
OBJECTIVE
The objective of this study was to synthesize and characterize 3D printed mesoporous bioactive glass (MBG), BG 45S5, and compare to β-Tricalcium phosphate (β-TCP) based scaffolds; test cell viability and osteogenic differentiation on human osteoprogenitor cells in vitro.
METHODS
MBG, BG 45S5, and β-TCP were fabricated into colloidal gel suspensions, tested with a rheometer, and manufactured into scaffolds using a 3D direct-write micro-printer. The materials were characterized in terms of microstructure and composition with Thermogravimetric Analyzer/Differential Scanning Calorimeter (TGA/DSC), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Micro-Computed Tomography (μ-CT), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDS), and Mattauch-Herzog-Inductively Coupled Plasma-Mass Spectrometry (MH-ICP-MS).
RESULTS
Scaffolds were tested for cell proliferation and osteogenic differentiation using human osteoprogenitor cells. Osteogenic media was used for differentiation, and immunocytochemistry for osteogenic markers Runx-2, Collagen-I, and Osteocalcin. The cell viability results after 7 days of culture yielded significantly higher (p < 0.05) results in β-TCP scaffolds compared to BG 45S5 and MBG groups.
CONCLUSION
All materials expressed osteogenic markers after 21 days of culture in expansion and osteogenic media.
背景
虽然自体移植物迄今为止仍是骨缺损填充物的“金标准”，但由于其物理和化学性质可定制等优势，合成骨移植物受到了关注。生物活性玻璃（BG）是一种无机材料，能够通过形成类似骨的磷灰石表面形成强键，从而增强成骨作用。与增材制造（3D 打印）相结合，有可能最大限度地发挥 BG 的骨再生特性。
目的
本研究旨在合成和表征 3D 打印介孔生物活性玻璃（MBG）、BG 45S5，并与β-磷酸三钙（β-TCP）基支架进行比较；测试其在体外对人成骨祖细胞的细胞活力和成骨分化。
方法
将 MBG、BG 45S5 和 β-TCP 制成胶体凝胶悬浮液，用流变仪进行测试，并使用 3D 直接书写微打印机制成支架。采用热重分析仪/差示扫描量热法（TGA/DSC）、傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）、微计算机断层扫描（μ-CT）、扫描电子显微镜（SEM）、能谱（EDS）和马塔豪-赫哲感应耦合等离子体质谱法（MH-ICP-MS）对材料的微观结构和组成进行了表征。
结果
用人类成骨祖细胞对支架进行了细胞增殖和成骨分化测试。使用成骨培养基进行分化，并使用免疫细胞化学检测成骨标志物 Runx-2、胶原-I 和骨钙素。培养 7 天后的细胞活力结果显示，β-TCP 支架组的细胞活力明显高于 BG 45S5 和 MBG 组（p<0.05）。
结论
在扩展和成骨培养基中培养 21 天后，所有材料均表达成骨标志物。
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