Marić Ivana, Yang Liangliang, Li Xiufeng, Santiago Guillermo Monreal, Pappas Charalampos G, Qiu Xinkai, Dijksman Joshua A, Mikhailov Kirill, van Rijn Patrick, Otto Sijbren
Stratingh Institute, Centre for Systems Chemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen (The, Netherlands.
Dutch Polymer Institute, P. O. Box 902, 5600 AX, Eindhoven (The, Netherlands.
Angew Chem Int Ed Engl. 2023 Mar 27;62(14):e202216475. doi: 10.1002/anie.202216475. Epub 2023 Feb 24.
Dynamic covalent chemistry (DCC) has proven to be a valuable tool in creating fascinating molecules, structures, and emergent properties in fully synthetic systems. Here we report a system that uses two dynamic covalent bonds in tandem, namely disulfides and hydrazones, for the formation of hydrogels containing biologically relevant ligands. The reversibility of disulfide bonds allows fiber formation upon oxidation of dithiol-peptide building block, while the reaction between NH-NH functionalized C-terminus and aldehyde cross-linkers results in a gel. The same bond-forming reaction was exploited for the "decoration" of the supramolecular assemblies by cell-adhesion-promoting sequences (RGD and LDV). Fast triggered gelation, cytocompatibility and ability to "on-demand" chemically customize fibrillar scaffold offer potential for applying these systems as a bioactive platform for cell culture and tissue engineering.
动态共价化学（DCC）已被证明是在全合成体系中创造迷人分子、结构和新兴性质的宝贵工具。在此，我们报告了一个串联使用两种动态共价键（即二硫键和腙）的体系，用于形成含有生物相关配体的水凝胶。二硫键的可逆性使得二硫醇 - 肽构建块氧化时形成纤维，而NH - NH官能化的C末端与醛交联剂之间的反应则导致凝胶形成。通过促进细胞粘附的序列（RGD和LDV）对超分子组装体进行“修饰”也利用了相同的成键反应。快速触发凝胶化、细胞相容性以及“按需”化学定制纤维支架的能力为将这些体系应用于细胞培养和组织工程的生物活性平台提供了潜力。
