Zhu Mingtao, Yu Jun, Wang Rong, Zeng Yongxian, Kang Linfeng, Chen Zhiyin
College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi, 417000, China; Horticulture College, Hunan Agricultural University, Changsha, Hunan, 410128, China.
Horticulture College, Hunan Agricultural University, Changsha, Hunan, 410128, China.
Plant Physiol Biochem. 2023 Mar;196:370-380. doi: 10.1016/j.plaphy.2023.01.058. Epub 2023 Feb 3.
To explore the use of L-aspartic acid nano-calcium (nano-Ca) to reduce nectarine fruit-cracking, we sprayed the crack-susceptible nectarine cultivar 'Huaguang' [Prunus persica (L.) Batsch var. nectarina (Ait.) Maxim.] with nano-Ca. The results showed that nano-Ca could reduce the fruit-cracking percentage of nectarine by more than 20%. Nano-Ca was effective because it increased the calcium pectinate content of the peel, reduced the activity of cell-wall metabolic enzymes, and changed the peel structure and enhanced its toughness. We also found that nano-Ca enhanced calmodulin activity in leaves, upregulated key genes of sucrose synthesis in leaves and sucrose transport in stem phloem, and significantly increased the soluble sugar content in the fruit by more than 2%. In addition, Nano-Ca also enhanced calmodulin activity in peel and up-regulated key genes related to anthocyanin-synthesis, promoting anthocyanin accumulation in the peel. The result will lay a theoretical foundation for the physiological and molecular mechanisms of nectarine-cracking and its prevention.
为探究L-天冬氨酸纳米钙（纳米钙）在减少油桃裂果方面的应用，我们用纳米钙对易裂果的油桃品种‘华光’[Prunus persica (L.) Batsch var. nectarina (Ait.) Maxim.]进行了喷施。结果表明，纳米钙可使油桃的裂果率降低20%以上。纳米钙之所以有效，是因为它增加了果皮中果胶酸钙的含量，降低了细胞壁代谢酶的活性，改变了果皮结构并增强了其韧性。我们还发现，纳米钙增强了叶片中钙调蛋白的活性，上调了叶片中蔗糖合成关键基因以及茎韧皮部中蔗糖运输关键基因的表达，并使果实中的可溶性糖含量显著增加了2%以上。此外，纳米钙还增强了果皮中钙调蛋白的活性，上调了与花青素合成相关的关键基因的表达，促进了花青素在果皮中的积累。该结果将为油桃裂果及其预防的生理和分子机制奠定理论基础。
