Matsuda Naoki
Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
J Insect Physiol. 2023 Mar;145:104489. doi: 10.1016/j.jinsphys.2023.104489. Epub 2023 Feb 4.
Many insects exhibit diel rhythms in physiology and behavior, driven by an endogenous circadian clock. Although aphids are paradigmatic insects whose photoperiodic time measurement is based on a heavily damped circadian clock, there is a lack of empirical data on such a damped circadian clock. The present study investigated the temporal distribution of hatching and the temporal expression patterns of circadian clock genes in the pea aphid, Acyrthosiphon pisum under light-dark (LD) cycles and constant darkness (DD). Hatching occurred intensively in the early photophase, and this rhythm persisted under LD cycles, but damped under DD for a few days. Of the six clock genes analyzed, cyc showed a temporal change in expression under LD cycles, whereas this temporal change was lost under DD. These results suggest that the circadian clock of A. pisum is easily damped during the embryonic stage, supporting the heavily damped oscillator model in photoperiodic time measurement of aphids.
许多昆虫的生理和行为表现出昼夜节律，这是由内源性生物钟驱动的。尽管蚜虫是典型的昆虫，其光周期时间测量基于一个严重衰减的生物钟，但关于这种衰减生物钟的实证数据却很缺乏。本研究调查了豌豆蚜（Acyrthosiphon pisum）在光暗（LD）循环和持续黑暗（DD）条件下孵化的时间分布以及生物钟基因的时间表达模式。孵化在光照期早期集中发生，这种节律在LD循环下持续存在，但在DD条件下几天内就衰减了。在所分析的六个生物钟基因中，cyc在LD循环下表达呈现时间变化，而在DD条件下这种时间变化消失了。这些结果表明，豌豆蚜的生物钟在胚胎阶段很容易衰减，这支持了蚜虫光周期时间测量中的严重衰减振荡器模型。
