罗马尼亚家养猫中的顶复门血寄生虫。
Apicomplexan haemoparasites in domestic cats in Romania.
机构信息
Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Calea Mănăștur 3-5, 400372, Cluj-Napoca, Romania.
CDS‑9: Molecular Biology and Veterinary Parasitology Unit, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372, Cluj-Napoca, Romania.
出版信息
Parasit Vectors. 2023 Feb 6;16(1):56. doi: 10.1186/s13071-023-05683-7.
BACKGROUND
Apicomplexan haemoparasites are protozoans that infect a variety of domestic and wild animal species, as well as humans. Data regarding haemoprotozoans in domestic cats are limited; therefore, the aim of this study was to assess the occurrence of Babesia spp., Cytauxzoon spp., and Hepatozoon spp. in domestic cats in Romania using molecular tools.
METHODS
Blood samples from 371 domestic cats were screened for the presence of piroplasmids. All samples that yielded a visible band in agarose gels were subsequently tested by specific assays targeting the 18S rDNA of Babesia spp., Cytauxzoon spp., and Hepatozoon spp. Moreover, nested PCR assays targeting mitochondrial genes of Babesia spp. were used for screening of all Babesia spp. 18S rDNA-positive samples.
RESULTS
From the total number of sampled cats, 19.4% were positive in the PCR assay targeting piroplasmids. Babesia spp. were identified in 15.1% of cats, while 0.5% were positive for Hepatozoon spp. Molecular analyses confirmed the presence of Babesia canis. No samples were positive for Cytauxzoon spp.
CONCLUSIONS
The high infection rates of domestic cats with Babesia spp. and the need for species differentiation highlight the importance of mitochondrial genes as targets for molecular protocols.
背景
顶复门血孢子虫是感染多种家养和野生动物以及人类的原生动物。关于家猫血液原生动物的数据有限；因此，本研究旨在使用分子工具评估罗马尼亚家养猫中巴贝斯虫属、西氏泰勒虫属和肝孢虫属的发生情况。
方法
对 371 只家猫的血液样本进行了梨形虫属的存在检测。所有在琼脂糖凝胶中产生可见条带的样本随后通过针对巴贝斯虫属、西氏泰勒虫属和肝孢虫属的 18S rDNA 的特异性检测进行了测试。此外，针对巴贝斯虫属的线粒体基因的巢式 PCR 检测用于筛查所有巴贝斯虫属 18S rDNA 阳性样本。
结果
在所采样的猫总数中，19.4%的 PCR 检测针对梨形虫属呈阳性。15.1%的猫被鉴定出巴贝斯虫属，而 0.5%的猫对肝孢虫属呈阳性。分子分析证实了犬巴贝斯虫的存在。没有样本对西氏泰勒虫属呈阳性。
结论
家猫巴贝斯虫属的高感染率和需要进行物种分化，突出了线粒体基因作为分子方案靶标的重要性。
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