Tello-Díaz Cristina, Palau Marta, Muñoz Estela, Gomis Xavier, Gavaldà Joan, Fernández-Hidalgo Nuria, Bellmunt-Montoya Sergi
Department of Vascular and Endovascular Surgery, Hospital de la Santa Creu i Sant Pau, Institute of Biomedical Research (II-B Sant Pau), CIBER CV, Barcelona, Spain.
Universitat Autònoma de Barcelona (UAB), Departament de Cirurgia i Ciències Morfològiques, Barcelona, Spain.
Microbiol Spectr. 2023 Feb 7;11(2):e0393122. doi: 10.1128/spectrum.03931-22.
The aim of this study was to quantify biofilm formation by methicillin-susceptible Staphylococcus aureus (MSSA) on the surfaces of different types of commonly used vascular grafts. We performed an study with two clinical strains of MSSA (MSSA2 and MSSA6) and nine vascular grafts: Dacron (Hemagard), Dacron-heparin (Intergard heparin), Dacron-silver (Intergard Silver), Dacron-silver-triclosan (Intergard Synergy), Dacron-gelatin (Gelsoft Plus), Dacron plus polytetrafluoroethylene (Fusion), polytetrafluoroethylene (Propaten; Gore), Omniflow II, and bovine pericardium (XenoSure). Biofilm formation was induced in two phases: an initial 90-minute adherence phase and a 24-hour growth phase. Quantitative cultures were performed, and the results were expressed as log CFU per milliliter. The Dacron-silver-triclosan graft and Omniflow II were associated with the least biofilm formation by both MSSA2 and MSSA6. MSSA2 did not form a biofilm on the Dacron-silver-triclosan graft (0 CFU/mL), and the mean count on the Omniflow II graft was 3.89 CFU/mL (standard deviation [SD] 2.10). The mean count for the other grafts was 7.01 CFU/mL (SD 0.82). MSSA6 formed a biofilm on both grafts, with 2.42 CFU/mL (SD 2.44) on the Dacron-silver-triclosan graft and 3.62 CFU/mL (SD 2.21) on the Omniflow II. The mean biofilm growth on the remaining grafts was 7.33 CFU/mL (SD 0.28). The differences in biofilm formation on the Dacron-silver-triclosan and Omniflow II grafts compared to the other tested grafts were statistically significant. Our findings suggest that of the vascular grafts we studied, the Dacron-silver-triclosan and Omniflow II grafts might prevent biofilm formation by MSSA. Although further studies are needed, these grafts seem to be good candidates for clinical use in vascular surgeries at high risk of infections due to this microorganism. The Dacron silver-triclosan and Omniflow II vascular grafts showed the greatest resistance to methicillin-susceptible Staphylococcus aureus biofilm formation compared to other vascular grafts. These findings could allow us to choose the most resistant to infection prosthetic graft.
本研究的目的是量化甲氧西林敏感金黄色葡萄球菌（MSSA）在不同类型常用血管移植物表面形成生物膜的情况。我们使用两株MSSA临床菌株（MSSA2和MSSA6）以及九种血管移植物进行了一项研究：涤纶（Hemagard）、含肝素涤纶（Intergard heparin）、含银涤纶（Intergard Silver）、含银 - 三氯生涤纶（Intergard Synergy）、含明胶涤纶（Gelsoft Plus）、涤纶加聚四氟乙烯（Fusion）、聚四氟乙烯（Propaten；Gore）、Omniflow II以及牛心包（XenoSure）。生物膜形成分两个阶段诱导：初始90分钟的黏附阶段和24小时的生长阶段。进行了定量培养，结果以每毫升对数CFU表示。含银 - 三氯生涤纶移植物和Omniflow II与MSSA2和MSSA6形成的生物膜最少有关。MSSA2在含银 - 三氯生涤纶移植物上未形成生物膜（0 CFU/mL），Omniflow II移植物上的平均菌落数为3.89 CFU/mL（标准差[SD] 2.10）。其他移植物的平均菌落数为7.01 CFU/mL（SD 0.82）。MSSA6在这两种移植物上均形成了生物膜，含银 - 三氯生涤纶移植物上为2.42 CFU/mL（SD 2.44），Omniflow II上为3.62 CFU/mL（SD 2.21）。其余移植物上的生物膜平均生长量为7.33 CFU/mL（SD 0.28）。与其他测试移植物相比，含银 - 三氯生涤纶和Omniflow II移植物上生物膜形成的差异具有统计学意义。我们的研究结果表明，在我们研究的血管移植物中，含银 -三氯生涤纶和Omniflow II移植物可能会阻止MSSA形成生物膜。尽管需要进一步研究，但这些移植物似乎是因这种微生物而感染风险高的血管手术临床应用的良好候选者。与其他血管移植物相比，含银 - 三氯生涤纶和Omniflow II血管移植物对甲氧西林敏感金黄色葡萄球菌生物膜形成表现出最大抗性。这些发现可以让我们选择最具抗感染性的人工移植物。
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