Hernández-Richter T, Schardey H M, Wittmann F, Mayr S, Schmitt-Sody M, Blasenbreu S, Heiss M M, Gabka C, Angele M K
Chirurgische Klinik und Poliklinik, Ludwig Maximilians-University, Munich, Germany.
Eur J Vasc Endovasc Surg. 2003 Nov;26(5):550-7. doi: 10.1016/s1078-5884(03)00344-7.
To evaluate the efficacy of silver- or Triclosan-coated prosthetic material compared to Rifampin bonded Dacron concerning their resistance to infection following subcutaneous implantation and contamination with Staphylococcus aureus.
Animal experimental study in mice.
Thirty-six C3H/HcN mice (Charles River Lab., Sulzfeld, Germany) with a weight between 24 and 27 g were randomised into six groups counting six animals each. Group I: control, gel-sealed dacron graft, group II: gel-sealed dacron graft and local contamination, group III: Intergard-Silver-prosthesis and contamination, group IV: silver/gel-sealed dacron prosthesis (test graft) and contamination, group V: Rifampin-bonded gel-sealed graft and contamination, group VI: Triclosan/collagen-coated dacron graft and contamination. Dacron graft material 0.8x1 cm was subcutaneously implanted in mice. Local contamination with 2x10(7)/0.2 ml S. aureus ATCC 25923 was carried out in groups II to VI. On day 14 the animals were killed and the grafts were explanted. The microscopic, histologic and microbiological evaluation of the graft material and the perigraft tissue was performed.
In control group I no case of infection was detected. In group II, 6 of 6 animals showed infection. In group III (Intergard-Silver) and group IV (silver/gel-test graft) were 6 of 6, in group V (Rifampin) only 1 of 6 grafts and in group VI (Triclosan) 4 of 6 grafts were infected. The difference between the low rate of infection in group V (Rifampin) in comparison to the completely infected groups III and IV (Silver) as well as the control group II was significant. Treatment of grafts with Triclosan could prevent infection only in 1/3 of the cases in group IV.
Silver coating failed to prevent graft infection material. A potential antimicrobial property was evident for Triclosan whereas Rifampin-bonded grafts exhibit a significantly reduced infection rate. Thus, silver-coated vascular grafts cannot ensure protection from vascular graft infection.
评估与利福平结合的涤纶相比，涂有银或三氯生的假体材料在皮下植入并被金黄色葡萄球菌污染后对感染的抵抗力。
小鼠动物实验研究。
36只体重在24至27克之间的C3H/HcN小鼠（德国苏尔茨费尔德的查尔斯河实验室）被随机分为六组，每组6只动物。第一组：对照组，凝胶密封涤纶移植物；第二组：凝胶密封涤纶移植物并局部污染；第三组：Intergard-Silver假体并污染；第四组：银/凝胶密封涤纶假体（测试移植物）并污染；第五组：利福平结合凝胶密封移植物并污染；第六组：三氯生/胶原蛋白涂层涤纶移植物并污染。将0.8×1厘米的涤纶移植物材料皮下植入小鼠体内。第二组至第六组进行2×10⁷/0.2毫升金黄色葡萄球菌ATCC 25923的局部污染。在第14天处死动物并取出移植物。对移植物材料和移植物周围组织进行显微镜、组织学和微生物学评估。
第一对照组未检测到感染病例。第二组6只动物中有6只出现感染。第三组（Intergard-Silver）和第四组（银/凝胶测试移植物）6只中有6只，第五组（利福平）6只移植物中只有1只，第六组（三氯生）6只移植物中有4只被感染。与完全感染的第三组和第四组（银）以及对照组第二组相比，第五组（利福平）感染率低的差异具有显著性。用三氯生处理移植物仅能在第四组的1/3病例中预防感染。
银涂层未能防止移植物材料感染。三氯生具有明显的潜在抗菌特性，而利福平结合的移植物感染率显著降低。因此，银涂层血管移植物不能确保预防血管移植物感染。
