Clinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.
Beijing Key Laboratory of Urinary Cellular Molecular Diagnostics, Beijing, China.
Medicine (Baltimore). 2023 Feb 3;102(5):e32862. doi: 10.1097/MD.0000000000032862.
The insulin signaling pathway plays an important role in the development of diabetes mellitus. The expression of insulin signaling pathway related proteins in the urine of diabetic patients has not been reported. The aim of this study was to analyze and verify the expression of insulin signaling pathway related proteins in the urine of diabetic patients without hypertension and hyperlipidemia, and to explore their clinical application value. Based on data-independent acquisition proteomics technology and bioinformatics, the urinary protein expression profile of diabetic patients without hypertension and hyperlipidemia was established. Western blot and enzyme-linked immunoassay were performed to verify the expression of insulin signaling pathway related proteins in the urine of diabetic patients. Sixteen proteins related to the insulin signaling pathway were screened in urine, and 7 of them were differentially expressed in the urine of diabetic patients without hypertension and hyperlipidemia. Further quantitative analysis showed that the downregulation of protein kinase CAMP-dependent type II regulatory subunit α, growth factor receptor bound protein 2, and guanine nucleotide-binding protein G(s) in the urine of diabetic patients without hyperlipidemia and hypertension was consistent with the preliminary screening results. In this exploratory study, we detected the expression of insulin signaling pathway related proteins in the urine of diabetic patients without hypertension and hyperlipidemia. protein kinase CAMP-dependent type II regulatory subunit α, growth factor receptor bound protein 2, and guanine nucleotide-binding protein G(s) in the urine of diabetic patients were downregulated, which was associated with diabetes. They may be promising noninvasive biomarkers for monitoring diabetes.
胰岛素信号通路在糖尿病的发生发展中起着重要作用。目前尚未有报道糖尿病患者尿中胰岛素信号通路相关蛋白的表达情况。本研究旨在分析和验证无高血压、高血脂的糖尿病患者尿中胰岛素信号通路相关蛋白的表达情况，并探讨其临床应用价值。本研究采用非依赖数据采集蛋白质组学技术和生物信息学方法，建立了无高血压、高血脂的糖尿病患者尿蛋白表达谱。通过 Western blot 和酶联免疫吸附试验对糖尿病患者尿中胰岛素信号通路相关蛋白的表达进行验证。在尿中筛选出 16 种与胰岛素信号通路相关的蛋白，其中 7 种在无高血压、高血脂的糖尿病患者尿中差异表达。进一步的定量分析显示，无高血压、高血脂的糖尿病患者尿中蛋白激酶 CAMP 依赖性 II 型调节亚基 α、生长因子受体结合蛋白 2 和鸟苷酸结合蛋白 G(s)下调，与初步筛选结果一致。在本探索性研究中，我们检测了无高血压、高血脂的糖尿病患者尿中胰岛素信号通路相关蛋白的表达情况。蛋白激酶 CAMP 依赖性 II 型调节亚基 α、生长因子受体结合蛋白 2 和鸟苷酸结合蛋白 G(s)在糖尿病患者尿中下调，与糖尿病有关，它们可能是有前途的非侵入性监测糖尿病的生物标志物。
