Department of Civil and Environmental Engineering, Faculty of Science and Technology, University of Macau, Macau 999078, China.
Institute of Environmental Research at Greater Bay Area, Key Laboratory for Water Quality and Conservation of the Pearl River De lta, Ministry of Education, Guangzhou University, Guangzhou 510006, China.
Environ Sci Technol. 2023 Feb 21;57(7):2804-2812. doi: 10.1021/acs.est.2c08059. Epub 2023 Feb 7.
Nanoplastics (NPs, <1 μm) are of great concern worldwide because of their high potential risk toward organisms in aquatic systems, while very little work has been focused on their tissue-specific toxicokinetics due to the limitations of NP quantification for such a purpose. In this study, NPs with two different sizes (86 and 185 nm) were doped with palladium (Pd) to accurately determine the uptake and depuration kinetics in various tissues (intestine, stomach, liver, gill, and muscle) of tilapia () in water, and subsequently, the corresponding toxic effects in the intestine were explored. Our results revealed uptake and depuration constants of 2.70-378 L kg day and 0.138-0.407 day for NPs in tilapia for the first time, and the NPs in tissues were found to be highly dependent on the particle size. The intestine exhibited the greatest relative accumulation of both sizes of NPs; the smaller NPs caused more severe damage than the larger NPs to the intestinal mucosal layer, while the larger NPs induced a greater impact on microbiota composition. The findings of this work explicitly indicate the size-dependent toxicokinetics and intestinal toxicity pathways of NPs, providing new insights into the ecological effects of NPs on aquatic organisms.
纳米塑料（NPs，<1μm）由于其对水生系统中生物的高潜在风险而受到全球关注，然而，由于缺乏用于此目的的 NP 定量方法，很少有工作关注它们的组织特异性毒代动力学。在本研究中，将两种不同尺寸（86 和 185nm）的 NPs 掺杂钯（Pd），以准确确定在水中罗非鱼（）的各种组织（肠、胃、肝、鳃和肌肉）中的摄取和清除动力学，随后探讨了相应的肠道毒性作用。我们的结果首次揭示了 NPs 在罗非鱼体内的摄取和清除常数分别为 2.70-378 L kg day 和 0.138-0.407 day，并且发现组织中的 NPs 高度依赖于粒径。两种尺寸的 NPs 在肠道中均表现出最大的相对积累；较小的 NPs 对肠黏膜层的损伤比较大的 NPs 更严重，而较大的 NPs 对微生物群落组成的影响更大。这项工作的结果明确表明了 NPs 的尺寸依赖性毒代动力学和肠道毒性途径，为 NPs 对水生生物的生态影响提供了新的见解。
