Joy R T, Hyneck M L, Berardi R R, Ho N F
Am J Hosp Pharm. 1979 May;36(5):618-21.
The effect of pH and time on the stability of heparin sodium in dextrose 5% in water (D5W) injection and in dextrose 5% in 0.45% sodium chloride injection was studied. Admixtures of heparin sodium 5,000 units/250 ml were tested after 0, 10, 20 and 30 minutes and 1, 2, 6, 12 and 24 hours of storage at room temperature. The pH of the carrier solutions was adjusted to 2, 4 or 9 prior to adding the heparin sodium. Heparin activity was measured using a thrombin clotting time assay. Samples were tested for pH changes at the same times. No substantial changes in heparin activity over the 24-hour period occurred with any of the pH-adjusted solutions. The pH of the heparin-D5W admixtures remained constant over time. The two carrier solutions, over a pH range of 2 to 9, appear to be suitable vehicles for heparin sodium.
研究了pH值和时间对肝素钠在5%葡萄糖注射液（D5W）以及5%葡萄糖与0.45%氯化钠注射液中的稳定性的影响。将5000单位/250ml的肝素钠混合物在室温下储存0、10、20和30分钟以及1、2、6、12和24小时后进行测试。在加入肝素钠之前，将载体溶液的pH值调节至2、4或9。使用凝血酶凝血时间测定法测量肝素活性。同时对样品进行pH值变化测试。在24小时内，任何pH调节溶液中的肝素活性均未发生实质性变化。肝素-D5W混合物的pH值随时间保持恒定。在pH值为2至9的范围内，这两种载体溶液似乎都是肝素钠的合适载体。
