Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macau SAR, China; MOE Frontiers Science Center for Precision Oncology, University of Macau, Macau SAR, China.
Department of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Thromb Res. 2023 Mar;223:146-154. doi: 10.1016/j.thromres.2023.01.032. Epub 2023 Feb 3.
Due to the delayed and vague symptoms, it is difficult to early diagnose mesenteric ischemia injuries in the dynamics of acute illness, leading to a 60-80 % mortality rate. Here, we found plasma fluorescence spectra can rapidly assess the severity of mesenteric ischemia injury in animal models. Ischemia-reperfusion damage of the intestine leads to multiple times increase in NADH, flavins, and porphyrin auto-fluorescence of blood. The fluorescence intensity ratio between blue-fluorophores and flavins can reflect the occurrence of shock. Using liquid chromatography and mass spectroscopy, we confirm that riboflavin is primarily responsible for the increased flavin fluorescence. Since humans absorb riboflavin from the intestine, its increase in plasma may indicate intestinal mucosa injury. Our work suggests a self-calibrated and reagent-free approach to identifying the emergence of fatal mesenteric ischemia in emergency departments or intensive care units.
由于症状延迟且不明显，在急性疾病的动态中很难早期诊断肠系膜缺血损伤，导致 60-80%的死亡率。在这里，我们发现血浆荧光光谱可以快速评估动物模型中肠系膜缺血损伤的严重程度。肠的缺血再灌注损伤导致 NADH、黄素和卟啉的血液自体荧光多次增加。蓝色荧光团与黄素之间的荧光强度比可以反映休克的发生。使用液相色谱和质谱，我们证实核黄素是导致黄素荧光增加的主要原因。由于人类从肠道吸收核黄素，其血浆中的增加可能表明肠黏膜损伤。我们的工作表明，有一种无需校准和试剂的方法可以识别急诊科或重症监护病房中致命性肠系膜缺血的发生。
