Liu Min, Fu Xiaojuan, Lu Mengjie, Liu Jijian, Xie Huihui, Wei Peng, Zhang Weidong, Xie Yahong, Qi Ying
MOE Key Laboratory of Oil and Gas Fine Chemicals, School of Chemical Engineering and Technology, Xinjiang University, Urumqi, 830046, China.
MOE Key Laboratory of Oil and Gas Fine Chemicals, School of Chemistry, Xinjiang University, Urumqi, 830046, China.
Anal Biochem. 2023 Apr 1;666:115077. doi: 10.1016/j.ab.2023.115077. Epub 2023 Feb 7.
The gold nanobipyramids (Au NBPs) are widely used in the analytical detection of biochemistry due to their unique localized surface plasmon resonance (LSPR) properties. In our developed approach, I in kelp was detected by etching Au NBPs in the presence of IO. Under acidic conditions, IO reacted rapidly with I to form I, subsequently I reacted with I to form the intermediate I. In the presence of CTAB, Au NBPs were etched by I derived from I, resulting in a decrease in the aspect ratio of Au NBPs, to form a significant blue shift of LSPR longitudinal peak and color variation of colloid which changed from blue-green to magenta and could be employed to quantitatively detect the concentration of I with the naked eye. A linear relationship can be found between the LSPR peak changes with the I concentration in a wide range from 4.0 μM to 15.0 μM, and the sensitive limit of detection (LOD) was 0.2 μM for UV-vis spectroscopy and the obvious color changes with a visual LOD was 4.0 μM for the naked eye. Benefiting from the high specificity, the proposed colorimetric detection of I in kelp samples was achieved, indicating the available potential of the colorimetric detection for the determination of I in real samples. What's more, this detection procedure was time-saving and could avoid tedious procedures.
金纳米双锥体（Au NBPs）因其独特的局域表面等离子体共振（LSPR）特性而被广泛应用于生物化学分析检测。在我们开发的方法中，通过在IO存在的情况下蚀刻Au NBPs来检测海带中的I。在酸性条件下，IO与I迅速反应形成I，随后I与I反应形成中间体I。在CTAB存在的情况下，Au NBPs被I衍生的I蚀刻，导致Au NBPs的纵横比降低，形成LSPR纵向峰的显著蓝移以及胶体颜色从蓝绿色变为品红色的变化，并且可以用于肉眼定量检测I的浓度。在4.0 μM至15.0 μM的宽范围内，LSPR峰变化与I浓度之间可以发现线性关系，紫外可见光谱的检测限（LOD）为0.2 μM，肉眼明显颜色变化的视觉LOD为4.0 μM。受益于高特异性，实现了海带样品中I的比色检测，表明比色检测在实际样品中测定I的潜在可用性。此外，该检测程序节省时间，可避免繁琐的程序。
