A statistical model to optimize indirect sandwich enzyme-linked immunosorbent assay parameters of antigen and antibody: a microcomputer program.

A new computer software program "AVCRV" was developed using a statistical model to analyze the data from the indirect sandwich enzyme-linked immunosorbent assay (ELISA). The software program calculates a sigmoid type of regression analysis and can be run on most microcomputers in the laboratory. The program permits those who are not familiar with computers to complete this type of analysis in a few seconds without a large mainframe computer or complicated software. This statistical model for a sigmoid type of regression analysis of ELISA data may improve the analysis of research data for various avian pathogens from several different experiments.