来自经干扰素-β处理的Daudi细胞的一种膜蛋白会导致细胞生长停止。

A membrane protein from IFN-beta-treated Daudi cells causes a cessation in cell growth.

作者信息

Hillman M C, Knight E, Blomstrom D C

机构信息

Central Research & Development Department, E. I. du Pont de Nemours and Company, Wilmington, DE 19898.

出版信息

Biochem Biophys Res Commun. 1987 Oct 14;148(1):140-7. doi: 10.1016/0006-291x(87)91087-4.

DOI:10.1016/0006-291x(87)91087-4

PMID:3675571

Abstract

An interferon-induced 17 kDa protein has been partially purified from the membranes of interferon-beta-treated Daudi cells. A fraction containing the 17 kDa protein purified 200 fold causes an inhibition of growth of Daudi, Namalva and Hela cells. The same fraction purified from the membranes of untreated cells causes no inhibition of cell growth. This interferon-beta-induced protein is located on the exterior of Daudi cells for it can be labeled with [125I] iodine catalyzed by lactoperoxidase. These results suggest that interferons induce a cell surface protein whose role is to cause an inhibition of cell growth.

摘要

一种干扰素诱导的17 kDa蛋白已从经β-干扰素处理的Daudi细胞膜中部分纯化出来。含有纯化200倍的17 kDa蛋白的组分可抑制Daudi、Namalva和Hela细胞的生长。从未经处理的细胞膜中纯化得到的相同组分对细胞生长无抑制作用。这种β-干扰素诱导的蛋白位于Daudi细胞的外部，因为它可以被乳过氧化物酶催化用[125I]碘标记。这些结果表明，干扰素诱导一种细胞表面蛋白，其作用是抑制细胞生长。