Chen Xiao Jiang, Liu Wen Zhao, Song Lin, Liu Hai Xia
College of Fisheries Science and Technology, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou City, Jiangsu Province, P. R. China.
College of Fisheries and Life Science, Dalian Ocean University, Dalian City, Liaoning Province, P. R. China.
Mitochondrial DNA B Resour. 2023 Feb 3;8(2):187-191. doi: 10.1080/23802359.2022.2151831. eCollection 2023.
The complete mitochondrial DNA genome of interruptus was sequenced on the Illumina HiSeq platform and found to be 16,735 bp and included 37 genes encoding 13 proteins, 22 tRNAs, two rRNAs, and two non-coding regions. The proportion of nucleotides in mitochondrial genome was T (27.9%), C (23.7%), A (33%), G (15.4%), and the deviation of AT was 60.9%. A Maximum-Likelihood phylogenetic tree was reconstructed using the concatenated mitochondrial protein-coding genes of and other 18 species of fishes. Phylogenetic analysis results supported that was closely related to . Fundamental genetic data of will be essential for further genetic studies.
对中断种的完整线粒体DNA基因组在Illumina HiSeq平台上进行了测序,发现其长度为16,735碱基对,包含37个基因,编码13种蛋白质、22个tRNA、2个rRNA和2个非编码区。线粒体基因组中核苷酸的比例为T(27.9%)、C(23.7%)、A(33%)、G(15.4%),AT偏差为60.9%。使用中断种和其他18种鱼类的串联线粒体蛋白质编码基因重建了最大似然系统发育树。系统发育分析结果支持中断种与[某物种]密切相关。中断种的基础遗传数据对于进一步的遗传研究至关重要。