Van Wormhoudt A, Fouchereau-Peron M
Laboratoire de Biologie Marine, Collège de France, Concarneau, France.
Biochem Biophys Res Commun. 1987 Oct 14;148(1):463-70. doi: 10.1016/0006-291x(87)91134-x.
Anti-salmon calcitonin antibodies were used to follow the purification of a high molecular weight peptide present both in the haemolymph and in the hepatopancreas of the Norway lobster Nephrops norvegicus. An apparent molecular weight of 22 kDa has been measured in electrophoresis on SDS gels and amino acid composition compared to salmon calcitonin. The amount determined by the immunoreactivity assay corresponds to about 1/40 and 1/140 of that which is based on direct protein measurement for the hepatopancreas and the haemolymph respectively. The total amount of this peptide could be estimated as 3.5 mg/g fresh weight for the hepatopancreas and 140 ug/ml for the haemolymph. The function of this peptide is still unknown.
抗鲑鱼降钙素抗体被用于追踪一种存在于挪威龙虾（Nephrops norvegicus）血淋巴和肝胰腺中的高分子量肽的纯化过程。在SDS凝胶电泳中测得其表观分子量为22 kDa，并与鲑鱼降钙素进行了氨基酸组成比较。免疫反应性测定所确定的量分别相当于肝胰腺和血淋巴中基于直接蛋白质测量量的约1/40和1/140。该肽的总量估计肝胰腺为3.5 mg/g鲜重，血淋巴为140 μg/ml。此肽的功能仍然未知。
