Chudan Seita, Ishibashi Riko, Nishikawa Miyu, Tabuchi Yoshiaki, Nagai Yoshinori, Ikushiro Shinichi, Furusawa Yukihiro
Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Kurokawa, Toyama 939-0398, Japan.
Department of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Toyama 939-0398, Japan.
Food Funct. 2023 Feb 21;14(4):2188-2199. doi: 10.1039/d2fo03396h.
Soluble oat fibers, including β-glucan, have been shown to alter the gut microbiome composition and ameliorate DSS-induced colitis; however, the beneficial effect of soluble oat fiber on colonic inflammation is not yet fully understood. In this study, we demonstrated that soluble oat fibers ameliorate T cell-dependent colitis through the induction of peripherally induced regulatory T cells (pTregs). Soluble oat fibers elevated colonic butyrate production dose-dependently, which coincided with the overrepresentation of (an analog of butyrate-producing ) in the gut microbiome. Soluble oat fibers promoted the growth of and even , and increased the concentration of butyrate in the culture supernatant. These results indicate that soluble oat fibers are an energy source for butyrate-producing bacteria and are a fermentation substrate. Soluble oat fibers increased the percentage of colonic pTregs and ameliorated the weight loss and inflammation in acute 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis; this may in part be mediated by the increase in IL-10-producing T cells. In conclusion, our results suggest that the administration of soluble oat fibers is a promising prebiotic treatment for the prevention of colitis mediated altered gut microbiota composition and elevated butyrate production.
包括β-葡聚糖在内的可溶性燕麦纤维已被证明可改变肠道微生物群组成并改善葡聚糖硫酸钠（DSS）诱导的结肠炎；然而，可溶性燕麦纤维对结肠炎症的有益作用尚未完全了解。在本研究中，我们证明可溶性燕麦纤维通过诱导外周诱导调节性T细胞（pTregs）来改善T细胞依赖性结肠炎。可溶性燕麦纤维剂量依赖性地提高结肠丁酸产量，这与肠道微生物群中丁酸产生菌（丁酸产生菌的类似物）的过度增殖相一致。可溶性燕麦纤维促进丁酸产生菌的生长，甚至增加培养上清液中丁酸的浓度。这些结果表明，可溶性燕麦纤维是丁酸产生菌的能量来源，是一种发酵底物。可溶性燕麦纤维增加了结肠pTregs的百分比，并改善了急性2,4,6-三硝基苯磺酸（TNBS）诱导的结肠炎中的体重减轻和炎症；这可能部分是由产生白细胞介素-10的T细胞增加介导的。总之，我们的结果表明，给予可溶性燕麦纤维是一种有前景的益生元治疗方法，可预防由肠道微生物群组成改变和丁酸产量升高介导的结肠炎。
