National Institute of Animal Health, NARO, 3-1-5 Kannondai, Tsukuba, Ibaraki, 305-0856, Japan.
United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu, 501-1193, Japan.
Arch Virol. 2023 Feb 9;168(3):84. doi: 10.1007/s00705-023-05709-6.
Virulent fowl adenovirus serotype 4 (FAdV-4) causes hydropericardium syndrome (HPS) with high mortality in chickens, leading to significant economic losses to the poultry industry. The development of an effective vaccine is essential for successful disease control. Here, we produced recombinant fiber-1 protein of FAdV-4, isolated from a Japanese HPS outbreak strain, JP/LVP-1/96, using a baculovirus expression system and evaluated its immunogenicity and protective efficacy. Recombinant fiber-1 protein induced high levels of neutralizing antibodies in immunized chickens, which were maintained for a minimum of 10 weeks. After being challenged with the virulent FAdV-4 strain JP/LVP-1/96, the immunized chickens did not exhibit clinical signs of infection or histopathological changes, there was a significant reduction in the viral load in various organs and total serum proteins, and albumin levels did not decline. These results suggest that the recombinant fiber-1 protein produced in this study can serve as a subunit vaccine to control HPS in chickens.
禽腺病毒 4 型（FAdV-4）是一种强毒型病毒，可引起鸡的包涵体肝炎（HPS），死亡率很高，给家禽业造成了重大经济损失。开发有效的疫苗对于成功控制疾病至关重要。本研究利用杆状病毒表达系统，制备了源自日本 HPS 暴发株 JP/LVP-1/96 的 FAdV-4 重组纤维-1 蛋白，并评估了其免疫原性和保护效力。重组纤维-1 蛋白可诱导免疫鸡产生高水平的中和抗体，且至少能维持 10 周。用强毒 FAdV-4 株 JP/LVP-1/96 攻毒后，免疫鸡未出现感染的临床症状或组织病理学变化，各器官中的病毒载量显著降低，总血清蛋白和白蛋白水平也未下降。这些结果表明，本研究制备的重组纤维-1 蛋白可作为亚单位疫苗用于控制鸡的 HPS。
