Department of Anesthesiology, Faculty of Medicine, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany.
Institute of Experimental Medicine and Systems Biology, Faculty of Medicine, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany.
Eur Radiol Exp. 2023 Feb 9;7(1):6. doi: 10.1186/s41747-022-00319-4.
Coronary physiology assessment in rodents by ultrasound is an excellent noninvasive and easy to perform technique, including pulsed-wave Doppler (PWD) and myocardial contrast echocardiography (MCE). Both techniques and the corresponding calculated parameters were investigated in this study at rest as well as their response to pharmacologically induced stress.
Left ventricular myocardial function was assessed in eight anaesthetised rats using transthoracic echocardiography. Coronary physiology was assessed by both PWD of the left coronary artery and MCE using a bolus method. Measurements were performed at rest and under stimulation with adenosine and dobutamine. Effects of stimulation on the calculated parameters were evaluated and rated by effect size (η).
Changes could be demonstrated by selected parameters of PWD and MCE. The clearest effect in PWD was found for diastolic peak velocity (η = 0.58). It increased from 528 ± 110 mm/s (mean ± standard deviation) at rest to 839 ± 342 mm/s (p = 0.001) with adenosine and 1093 ± 302 mm/s with dobutamine (p = 0.001). The most distinct effect from MCE was found for the normalised wash-in rate (η = 0.58). It increased from 1.95 ± 0.35% at rest to 3.87 ± 0.85% with adenosine (p = 0.001) and 3.72 ± 1.03% with dobutamine (p = 0.001).
Induced changes in coronary physiology by adenosine and dobutamine could successfully be monitored using MCE and PWD in anaesthetised rats. Due to the low invasiveness of the measurements, this protocol could be used for longitudinal animal studies.
通过超声对啮齿动物进行冠状动脉生理学评估是一种出色的非侵入性且易于执行的技术，包括脉冲波多普勒（PWD）和心肌对比超声心动图（MCE）。本研究探讨了这两种技术及其相应的计算参数在休息状态下的表现，以及它们对药理学诱导应激的反应。
通过经胸超声心动图评估 8 只麻醉大鼠的左心室心肌功能。通过左冠状动脉的 PWD 和 MCE 中的造影剂团块法来评估冠状动脉生理学。在休息状态下和使用腺苷和多巴酚丁胺刺激下进行测量。通过效应量（η）评估刺激对计算参数的影响。
通过 PWD 和 MCE 的选定参数可以观察到变化。PWD 中最明显的影响是舒张峰值速度（η=0.58）。与休息时的 528±110mm/s（平均值±标准差）相比，腺苷刺激下增加至 839±342mm/s（p=0.001），多巴酚丁胺刺激下增加至 1093±302mm/s（p=0.001）。MCE 中最明显的影响是正常化的灌注率（η=0.58）。与休息时的 1.95±0.35%相比，腺苷刺激下增加至 3.87±0.85%（p=0.001），多巴酚丁胺刺激下增加至 3.72±1.03%（p=0.001）。
通过 MCE 和 PWD，成功地在麻醉大鼠中监测到腺苷和多巴酚丁胺诱导的冠状动脉生理学变化。由于测量的低侵入性，该方案可用于纵向动物研究。
