Elsaba Yasmin M, El-Hennawi Heba M, Ibrahim Mona M, Wehaidy Hala R
Botany and Microbiology Department, Faculty of Sciences, Helwan University, Cairo, Egypt.
Dyeing, Printing and Textile Auxiliaries Department, National Research Centre, Dokki, Giza, Egypt.
J Genet Eng Biotechnol. 2023 Feb 9;21(1):14. doi: 10.1186/s43141-023-00473-1.
Flavonoid natural dyes have gained attention because they are nontoxic and eco-friendly. However, they do not work effectively with artificial fibers and require the use of mordants, which are considered as hazardous chemicals. Laccase enzyme catalyzes the oxidation of phenols, forming phenoxyl radicals that undergo a further polymerization process. So, laccase can oxidize flavonoid dyes, and it can be used instead of harmful mordants in flavonoid dye fixation on cotton fabrics. Laccases also are involved in a variety of metabolic processes, and they have anti-proliferative effects toward HepG2 and MCF-7 tumor cells.
Among fifteen fungal isolates, the fungus Ceratorhiza hydrophila isolated from the submerged plant Myriophyllum spicatum was selected as the most potent laccase producer. Optimization of the production medium resulted in a 9.9-fold increase in laccase productivity. The partially purified Ceratorhiza hydrophila laccase could successfully improve the affinity of cotton fabrics toward quercetin (flavonoid) dye with excellent color fastness properties. The partially purified laccase also showed anti-proliferative activity against HepG2 and MCF-7 tumor cells. However, high laccase concentration is required to estimate IC50.
Ceratorhiza hydrophila MK387081 is an excellent laccase producer. The partially purified laccase from Ceratorhiza hydrophila can be used in textile dyeing and printing processes as a safer alternative to the conventional hazardous mordants. Also, it can be used in preparation of cancer treatment drugs. However, further studies are needed to investigate IC50 for both cell types at higher laccase concentrations.
黄酮类天然染料因其无毒且环保而受到关注。然而，它们对人造纤维不起作用，并且需要使用媒染剂，而媒染剂被认为是有害化学物质。漆酶催化酚类的氧化，形成酚氧基自由基，这些自由基会经历进一步的聚合过程。因此，漆酶可以氧化黄酮类染料，并且可以在棉织物上固定黄酮类染料时替代有害媒染剂。漆酶还参与多种代谢过程，并且对肝癌细胞系HepG2和乳腺癌细胞系MCF - 7具有抗增殖作用。
在15株真菌分离物中，从沉水植物狐尾藻分离得到的嗜水角喙孢菌被选为最有效的漆酶产生菌。对生产培养基进行优化后，漆酶生产力提高了9.9倍。部分纯化的嗜水角喙孢菌漆酶能够成功提高棉织物对槲皮素（黄酮类）染料的亲和力，并具有优异的色牢度性能。部分纯化的漆酶对肝癌细胞系HepG2和乳腺癌细胞系MCF - 7也显示出抗增殖活性。然而，需要高浓度的漆酶来测定半数抑制浓度（IC50）。
嗜水角喙孢菌MK387081是一种优良的漆酶产生菌。部分纯化的嗜水角喙孢菌漆酶可作为传统有害媒染剂的更安全替代品，用于纺织印染工艺。此外，它还可用于制备癌症治疗药物。然而，需要进一步研究在更高漆酶浓度下两种细胞类型的半数抑制浓度（IC50）。
