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纹石蛾科(毛翅目)的初步分子系统发育:靶向富集数据与传统序列数据结合的探索

A preliminary molecular phylogeny of the family Hydroptilidae (Trichoptera): an exploration of combined targeted enrichment data and legacy sequence data.

作者信息

Thomson Robin E, Frandsen Paul B, Holzenthal Ralph W

机构信息

University of Minnesota, Department of Entomology, 1980 Folwell Ave., St. Paul, MN 55108, USA University of Minnesota St. Paul United States of America.

Brigham Young University, Department of Plant and Wildlife Sciences, Provo, UT 84602, USA Brigham Young University Provo, UT United States of America.

出版信息

Zookeys. 2022 Jul 11;1111:467-488. doi: 10.3897/zookeys.1111.85361. eCollection 2022.

DOI:10.3897/zookeys.1111.85361
PMID:36760852
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9848688/
Abstract

Hydroptilidae is an extremely diverse family within Trichoptera, containing over 2,600 known species, that displays a wide array of ecological, morphological, and habitat diversity. However, exploration into the evolutionary history of microcaddisflies based on current phylogenetic methods is mostly lacking. The purpose of this study is to provide a proof-of-concept that the use of molecular data, particularly targeted enrichment data, and statistically supported methods of analysis can result in the construction of a stable phylogenetic framework for the microcaddisflies. Here, a preliminary exploration of the hydroptilid phylogeny is presented using a combination of targeted enrichment data for ca. 300 nuclear protein-coding genes and legacy (Sanger-based) sequence data for the mitochondrial COI gene and partial sequence from the 28S rRNA gene.

摘要

纹石蛾科是毛翅目昆虫中一个极其多样化的科,已知物种超过2600种,展现出广泛的生态、形态和栖息地多样性。然而,基于当前系统发育方法对微石蛾进化史的探索大多仍处于缺失状态。本研究的目的是提供一个概念验证,即使用分子数据,特别是靶向富集数据以及经过统计学支持的分析方法,能够构建一个稳定的微石蛾系统发育框架。在此,我们结合约300个核蛋白编码基因的靶向富集数据以及线粒体COI基因和28S rRNA基因部分序列的传统(基于桑格测序法)序列数据,对纹石蛾科的系统发育进行了初步探索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e8/9848688/269b47c9ef9b/zookeys-1111-467_article-85361__-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e8/9848688/c25a7f364f1f/zookeys-1111-467_article-85361__-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e8/9848688/0e55e7d8e374/zookeys-1111-467_article-85361__-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e8/9848688/269b47c9ef9b/zookeys-1111-467_article-85361__-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e8/9848688/c25a7f364f1f/zookeys-1111-467_article-85361__-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e8/9848688/0e55e7d8e374/zookeys-1111-467_article-85361__-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e8/9848688/269b47c9ef9b/zookeys-1111-467_article-85361__-g003.jpg

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