Chen Shimin, Luo Xuming, Nanda Satyabrata, Yang Chunxiao, Li Zhaoyang, Zhang Youjun, Zhou Xuguo, Pan Huipeng
Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Guangzhou 510642, China.
MS Swaminathan School of Agriculture, Centurion University of Technology and Management, Paralakhemundi 761200, India.
J Agric Food Chem. 2023 Feb 10. doi: 10.1021/acs.jafc.2c08473.
RNA interference (RNAi)-mediated control of the notorious pest is an emerging environment friendly research area. However, the characterization of key target genes in is crucial for this. Additionally, assessing the risk of RNAi to nontarget organisms (NTOs) is necessary for environmental safety. In this study, the potential of RNAi technology in controlling infestation has been investigated by the oral delivery of double-stranded RNA (dsRNA). The results revealed that the silencing of six genes, including , , , , , and , was highly lethal to . The LC values of the dsRNAs used to silence these six genes were found to be less than 13 ng/μL. Moreover, the use of the bacterially expressed dsRNAs caused high mortality in the lab and field populations of . Further, administration of and dsRNAs in the predatory lady beetle confirmed no transcriptional or organismal levels effects. This risk-assessment result ensured no off-target RNAi effects on the NTOs. Overall, the findings of the study suggested that , , , , , and can be novel promising molecular targets with high specificity for management with negligible effects on the NTOs.
RNA干扰（RNAi）介导的对这种臭名昭著害虫的控制是一个新兴的环境友好型研究领域。然而，确定该害虫的关键靶基因对于此至关重要。此外，评估RNAi对非靶标生物（NTOs）的风险对于环境安全是必要的。在本研究中，通过口服双链RNA（dsRNA）研究了RNAi技术在控制该害虫侵扰方面的潜力。结果显示，包括[具体基因1]、[具体基因2]、[具体基因3]、[具体基因4]、[具体基因5]和[具体基因6]在内的六个基因的沉默对该害虫具有高度致死性。用于沉默这六个基因的dsRNAs的LC值被发现小于13 ng/μL。此外，使用细菌表达的dsRNAs在该害虫的实验室和田间种群中导致了高死亡率。此外，在捕食性瓢虫[瓢虫名称]中施用[具体基因1]和[具体基因2]的dsRNAs证实没有转录或生物水平的影响。这一风险评估结果确保了对非靶标生物没有脱靶RNAi效应。总体而言，该研究结果表明，[具体基因1]、[具体基因2]、[具体基因3]、[具体基因4]、[具体基因5]和[具体基因6]可以成为对该害虫管理具有高特异性且对非靶标生物影响可忽略不计的新的有前景的分子靶标。
