Gautschi-Sova P, Jiang Z P, Fràter-Schröder M, Böhlen P
Department of Biochemistry, University of Zürich, Switzerland.
Biochemistry. 1987 Sep 8;26(18):5844-7. doi: 10.1021/bi00392a039.
Endothelial cell growth factor activity purified from bovine kidney by heparin-Sepharose affinity chromatography was previously identified as basic fibroblast growth factor [Baird, A., Esch, F., Böhlen, P., Ling, N., & Gospodarowicz, D. (1985) Regul. Pept. 12, 202-213]. We now show that a major mitogenic fraction, isolated from heparin-Sepharose-purified material by Mono-S cation-exchange chromatography and reverse-phase high-performance liquid chromatography, is related to acidic fibroblast growth factor (aFGF). Sequence analysis showed the amino-terminal sequence to be Tyr-Lys-Lys-Pro-Lys-Leu-Leu-Tyr-X-Ser-Asn-Gly-Gly-Tyr-Phe-Leu-Arg-Ile-Le u-Pro- Asp-Gly-Thr-Val-Asp-. The molecular mass of the protein, as determined by polyacrylamide gel electrophoresis, was 15.5 kDa. In combination, those data strongly suggest that this mitogen is amino terminally truncated acidic fibroblast growth factor. So far, aFGF has only been found in neural tissues, i.e., in the brain and retina. Our results strongly suggest that this mitogen also occurs in extraneural tissue.
先前通过肝素-琼脂糖亲和层析从牛肾中纯化得到的内皮细胞生长因子活性物质被鉴定为碱性成纤维细胞生长因子[贝尔德，A.，埃施，F.，博伦，P.，林，N.，&戈斯波达罗维茨，D.（1985年）调节肽12，202 - 213]。我们现在表明，通过单磺酸阳离子交换层析和反相高效液相色谱从肝素-琼脂糖纯化的物质中分离出的一种主要促有丝分裂组分与酸性成纤维细胞生长因子（aFGF）有关。序列分析表明其氨基末端序列为Tyr-Lys-Lys-Pro-Lys-Leu-Leu-Tyr-X-Ser-Asn-Gly-Gly-Tyr-Phe-Leu-Arg-Ile-Leu-Pro-Asp-Gly-Thr-Val-Asp-。通过聚丙烯酰胺凝胶电泳测定的该蛋白质的分子量为15.5 kDa。综合这些数据强烈表明这种促有丝分裂原是氨基末端截短的酸性成纤维细胞生长因子。到目前为止，aFGF仅在神经组织中被发现，即大脑和视网膜中。我们的结果强烈表明这种促有丝分裂原也存在于神经外组织中。
