Nakamura Y, Smith M, Krishna A, Terranova P F
Department of Physiology, Ralph L. Smith Research Center, University of Kansas Medical Center, Kansas City 66103.
Biol Reprod. 1987 Oct;37(3):546-9. doi: 10.1095/biolreprod37.3.546.
The number of mast cells per microscopic field at 450X (0.123 mm2) was determined in the bovine ovary on Days 4, 10, and 19 of the cycle (Day 0 = estrus). Mast cells were observed in the theca externa of follicles, the external capsule of the corpus luteum, and in the stromal and hilar regions of the ovary. The stromal and hilar regions exhibited the highest number of mast cells (per field = 7.51-10.15) compared with the follicular and luteal compartments (2.65-3.70 mast cells/field). Most interestingly, on Day 19 a significant increase in the number of mast cells was observed in the theca externa of the dominant follicle (10.4 mast cells/field) compared with nondominant healthy follicles on Days 4 and 10 (2-3/field). After ovulation, the number of mast cells surrounding the corpus luteum on Day 4 was significantly lower than 19 values in the dominant follicle; this was further diminished on Day 10 of the cycle. These results indicate that the increase in mast cells associated with the dominant follicle on Day 19 may be related to its development and the decline in mast cells related to ovulation.
在发情周期的第4天、第10天和第19天（第0天=发情期），测定牛卵巢在450倍放大倍数（0.123平方毫米）下每个显微镜视野中的肥大细胞数量。在卵泡的外膜、黄体的外膜以及卵巢的间质和门区观察到肥大细胞。与卵泡和黄体部分（每个视野2.65 - 3.70个肥大细胞）相比，间质和门区的肥大细胞数量最多（每个视野 = 7.51 - 10.15个）。最有趣的是，在第19天，与第4天和第10天的非优势健康卵泡（每个视野2 - 3个）相比，优势卵泡的外膜中肥大细胞数量显著增加（每个视野10.4个肥大细胞）。排卵后，第4天黄体周围的肥大细胞数量明显低于优势卵泡第19天的数量；在发情周期的第10天，这一数量进一步减少。这些结果表明，第19天与优势卵泡相关的肥大细胞增加可能与其发育有关，而与排卵相关的肥大细胞减少。
