Institute for Biochemistry and Molecular Biology, Department of Chemistry, Faculty of Mathematics, Computer Science and Natural Science, Hamburg University, Germany.
Biochem Biophys Res Commun. 2023 Apr 2;650:17-20. doi: 10.1016/j.bbrc.2023.01.028. Epub 2023 Feb 4.
Inevitable precondition for ribonucleic acids to regulate gene expression and to perform gene editing is diffusion. Free three-dimensional translational diffusion velocity of RNA of up to 200 nucleotides could be predicted with high accuracy by the empirical model D = 4.58 10 N ms. Furthermore, the biological function of ribonucleic acids is determined by rotational diffusion. In the presented work, an empirical model is derived applying atom-level shell-modeling of electron density maps, D = 1.62 10 N s, to predict the rotational diffusion coefficient of short ribonucleic acids based on the polymer size.
核糖核酸调控基因表达和进行基因编辑的必然前提是扩散。通过经验模型 D = 4.58×10^-17N·ms，可以高精度地预测长达 200 个核苷酸的 RNA 的自由三维平动扩散速度。此外，核糖核酸的生物学功能由旋转扩散决定。在本工作中，应用基于电子密度图的原子级壳模型，导出了一个经验模型，D = 1.62×10^-17N·s，以根据聚合物尺寸预测短核糖核酸的旋转扩散系数。
