Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States.
Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States; Department of Cancer Biology and Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States.
Methods Enzymol. 2023;681:215-240. doi: 10.1016/bs.mie.2022.10.006. Epub 2022 Dec 8.
Targeted protein degradation has emerged as a useful approach for both basic biological investigations and therapeutic development. However, it can provide confounding results if not properly controlled. In this manuscript, we discuss the importance of proper controls and provide a detailed protocol for their application to proteolysis targeting chimera mediated degradation.
靶向蛋白降解已成为基础生物学研究和治疗开发的一种有用方法。然而，如果没有得到妥善控制，它可能会提供混淆的结果。在本文中，我们讨论了正确控制的重要性，并提供了详细的方案，以将其应用于蛋白水解靶向嵌合体介导的降解。
