Neubauer A, Sauer H, Valet G
Klinikum Charlottenburg, FU-Berlin, Abteilung Innere Medizin.
Blut. 1987 Nov;55(5):433-45. doi: 10.1007/BF00367460.
Human bone marrow cells from 20 patients as well as the permanent human B-cell lines RPMI 1788, Raji, Daudi, T-cell lines Molt, CEM, Jurkat and the promyelocytic line HL 60 were assayed by means of a newly developed in vitro flow cytometric cytostatic drug assay. The cells were exposed to cytosine-arabinoside, L-asparaginase, daunorubicin, prednisone or vincristine. Surviving cells were stained after an incubation period of 2 to 7 days with esterase and pH-indicator dye ADB (1,4-diacetoxy-2,3-dicyanobenzene), dead cells with DNA-dye PI (propidium iodide). Dose-response curves were established using percent surviving cells. It was possible to evaluate bone marrow samples from 16 out of 20 patients. Seven samples were leukemic (acute myeloid leukemia (AML) n = 6, Non-Hodgkin's Lymphoma (NHL) n = 1). Nine samples were from patients either in complete remission or with benign diseases. Daunorubicin and cytosine-arabinoside were cytotoxic in both groups, whereas vincristine was effective mainly in the leukemic group (p less than 0.05). There was significant heterogeneity in the reactivity of AML-marrow cells from different patients to different drugs. The cell lines exhibited different patterns of sensitivity. Vincristine arrested cells in G2/M-phase, cytosine-arabinoside caused an increase of cells in the S-phase.
采用一种新开发的体外流式细胞仪细胞抑制药物分析法，对来自20例患者的人骨髓细胞以及永久性人B细胞系RPMI 1788、Raji、Daudi、T细胞系Molt、CEM、Jurkat和早幼粒细胞系HL 60进行了检测。将细胞暴露于阿糖胞苷、L-天冬酰胺酶、柔红霉素、泼尼松或长春新碱中。在2至7天的孵育期后，用酯酶和pH指示剂染料ADB（1,4-二乙酰氧基-2,3-二氰基苯）对存活细胞进行染色，用DNA染料PI（碘化丙啶）对死亡细胞进行染色。使用存活细胞百分比建立剂量反应曲线。对20例患者中的16例的骨髓样本进行了评估。7份样本为白血病样本（急性髓性白血病（AML）n = 6，非霍奇金淋巴瘤（NHL）n = 1）。9份样本来自完全缓解或患有良性疾病的患者。柔红霉素和阿糖胞苷在两组中均具有细胞毒性，而长春新碱主要在白血病组中有效（p小于0.05）。不同患者的AML骨髓细胞对不同药物的反应存在显著异质性。细胞系表现出不同的敏感性模式。长春新碱使细胞停滞在G2/M期，阿糖胞苷导致S期细胞增加。
