Valet G, Warnecke H H, Kahle H
Blut. 1984 Jul;49(1):37-43. doi: 10.1007/BF00320382.
A new assay for cytostatic drug testing is described which can be automated. Pleural effusions and ascites are cultured as such for one week. Cells of solid tumors are cultured in the patients own serum for the same time. The cells are then stained with the esterase and intracellular pH-indicator dye 1,4-diacetoxy-2,3-dicyano-benzene (ADB) to label vital cells. They are simultaneously stained with propidium iodide (PI) as an indicator for dead cells. Monosized fluorescent latex particles are added as concentration, volume and fluorescence standard. Inflammatory cells can be distinguished in the assay from tumor cells because of their small cell volume. The number of dead and surviving cells is counted by the flow cytometer and a therapeutic index is calculated as ratio between the surviving inflammatory to surviving tumor cells. An important feature of the assay is that the DNA-distribution of the dead cells (e.g. aneuploidy) as well as the functional state of the surviving tumor cells and inflammatory cells can be judged from intracellular esterase activity and intracellular pH.
描述了一种可自动化的用于细胞抑制药物测试的新检测方法。胸腔积液和腹水直接培养一周。实体瘤细胞在患者自身血清中培养相同时间。然后用酯酶和细胞内pH指示剂染料1,4 - 二乙酰氧基 - 2,3 - 二氰基苯（ADB）对细胞进行染色以标记活细胞。同时用碘化丙啶（PI）作为死细胞的指示剂进行染色。添加单尺寸荧光乳胶颗粒作为浓度、体积和荧光标准。在该检测中，炎症细胞因其细胞体积小而可与肿瘤细胞区分开来。通过流式细胞仪对死细胞和存活细胞进行计数，并计算治疗指数，即存活的炎症细胞与存活的肿瘤细胞的比例。该检测的一个重要特征是，可以从细胞内酯酶活性和细胞内pH判断死细胞的DNA分布（例如非整倍体）以及存活肿瘤细胞和炎症细胞的功能状态。
