[The Expression and Correlation of , and Calreticulin in Diffuse Large B-Cell Lymphoma].

OBJECTIVE

To analyze the expression and correlation of microRNA-195 (miR-195), and calreticulin in diffuse large B-cell lymphoma (DLBCL).

METHODS

From April 2020 to April 2021, 80 DLBCL patients with complete data archived by the Pathology Department of Handan First Hospital and The Second Hospital of Hebei Medical University were selected as the study group, and 70 patients with reactive lymph node hyperplasia were selected as the control group. The expressions of and were detected by real-time fluorescence quantitative PCR, and the expression of calreticulin was detected by Western blot. Pearson correlation was used to analyze the correlation between , , calreticulin and DLBCL, and ROC curve was used to analyze the predictive value of , and calreticulin for DLBCL.

RESULTS

Compared with the control group, the expression of decreased but and calreticulin increased in the study group (<0.001). The expression levels of , and calreticulin were not related to sex, age, primary site and B symptoms of patients with DLBCL, but related to immunophenotype, Ann Arbor stage, lactate dehydrogenase, IPI score, nodule involvement and Ki-67 index. The expression of decreased and the expression of and calreticulin increased in DLBCL paitents with non-germinal center source, Ann Arbor stage III-IV, lactate dehydrogenase > 245 U/L, IPI score 3-5, nodule involvement≥2 and Ki-67 index≥75% (<0.05). Pearson correlation analysis showed that and were negatively correlated (=-0.536, =0.001), and calreticulin were negatively correlated (=-0.545, =0.001), while and calreticulin were positively correlated (=0.523, =0.001). ROC curve showed that compared with the single diagnosis of , and calreticulin, the combination of the three items had higher predictive value for DLBCL (<0.001).

CONCLUSION

The expression of decreases and the expression of and calreticulin increase in patients with DLBCL. Along with the increase of disease stage and IPI score, the decrease of and the increase of and calreticulin aggravate gradually. The three items may participate in the occurrence and progress of DLBCL.