Department of Life and Environmental Sciences, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
Department of Clinical and Molecular Sciences (DISCLIMO), Università Politecnica delle Marche, Via Tronto 10/a, 60126 Ancona, Italy.
Cells. 2023 Jan 19;12(3):364. doi: 10.3390/cells12030364.
Several in vivo trials have previously demonstrated the beneficial effects of the administration of various probiotic forms on bone health. In this study, we explored the potency of two probiotics, and , alone or in combination with vitamin D (VD), to modulate the transcription of genes involved in the ossification process in a human osteoblast cell line. Genes that mark the "osteoblast proliferation phase", such as , , and , "extracellular matrix (ECM) maturation", such as and , as well as "ECM mineralization", such as , , and , were all highly expressed in osteoblasts treated with extract. The observed increase in the transcription of the mRNA was further in agreement with its protein levels as observed by Western blot and immunofluorescence. Therefore, this higher transcription and translation of alkaline phosphatase in osteoblasts treated with the extract, indicated its substantial osteogenic impact on human osteoblasts. Although both the probiotic extracts showed no osteogenic synergy with VD, treatment with alone could increase the ECM mineralization, outperforming the effects of and even VD. Furthermore, these results supported the validity of employing probiotic extracts rather than live cells to investigate the effects of probiotics in the in vitro systems.
先前已有几项体内试验证明了各种益生菌形式的管理对骨骼健康的有益作用。在这项研究中，我们探索了两种益生菌， 和 ，单独或与维生素 D（VD）联合使用，以调节人成骨细胞系中参与骨化过程的基因转录。标记“成骨细胞增殖阶段”的基因，如 、 和 ，“细胞外基质（ECM）成熟”，如 和 ，以及“ECM 矿化”，如 、 和 ，在经 提取物处理的成骨细胞中均高度表达。通过 Western blot 和免疫荧光观察到的 基因转录增加进一步证实了这一点。因此，在用 提取物处理的成骨细胞中碱性磷酸酶的转录和翻译增加表明其对人成骨细胞具有实质性的成骨作用。尽管两种益生菌提取物与 VD 均无协同成骨作用，但单独使用 即可增加 ECM 矿化，效果优于 和 VD。此外，这些结果支持使用益生菌提取物而不是活细胞来研究益生菌在体外系统中的作用的有效性。
