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从 pv. 中克隆和鉴定出 5-羟色胺-乙酰基转移酶基因 。

Molecular Cloning and Characterization of a Serotonin -Acetyltransferase Gene, , from pv. .

机构信息

State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection, Ministry of Agriculture and Rural Affairs, Zhejiang Provincial Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.

Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China.

出版信息

Int J Environ Res Public Health. 2023 Jan 19;20(3):1865. doi: 10.3390/ijerph20031865.

DOI:10.3390/ijerph20031865
PMID:36767232
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9914633/
Abstract

Rice bacterial blight (BB), caused by pv. (), is one of the top ten bacterial plant diseases worldwide. Serotonin -acetyltransferase (SNAT) is one of the key rate-limiting enzymes in melatonin (MT) biosynthesis. However, its function in pathogenic bacteria remains unclear. In this study, a SNAT protein (xoSNAT3) that showed 27.39% homology with sheep SNAT was identified from a collection of 24 members of GCN5-related -acetyltransferase (GNAT) superfamily in . This xoSNAT3 could be induced by MT. In tobacco-based transient expression system, xoSNAT3 was found localized on mitochondria. In vitro studies indicated that xoSNAT3 showed the optima enzymatic activity at 50 °C. The recombinant enzyme showed K and values of 709.98 μM and 2.21 nmol/min/mg protein, respectively. Mutant △ showed greater impaired MT biosynthesis than the wild-type strain. Additionally, △ showed 14.06% less virulence and 26.07% less biofilm formation. Collectively, our results indicated that services as a SNAT involved in MT biosynthesis and pathogenicity in .

摘要

水稻细菌性条斑病（BB）由 pv. ()引起，是全球十大细菌性植物病害之一。血清素-乙酰基转移酶（SNAT）是褪黑素（MT）生物合成中的关键限速酶之一。然而，其在致病细菌中的功能尚不清楚。本研究从 pv. 的 GCN5 相关乙酰基转移酶（GNAT）超家族的 24 个成员中鉴定出一种与绵羊 SNAT 具有 27.39%同源性的 SNAT 蛋白 (xoSNAT3)。该 xoSNAT3 可被 MT 诱导。在烟草瞬时表达系统中，xoSNAT3 定位于线粒体上。体外研究表明，xoSNAT3 在 50°C 时表现出最佳的酶活性。重组酶的 K 和 值分别为 709.98 μM 和 2.21 nmol/min/mg 蛋白。突变体 △比野生型菌株表现出更大的 MT 生物合成受损。此外，△的毒力降低了 14.06%，生物膜形成减少了 26.07%。综上所述，我们的结果表明， xoSNAT3 在 pv. 中作为参与 MT 生物合成和致病性的 SNAT。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d223/9914633/889f5f3a6ce8/ijerph-20-01865-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d223/9914633/7731566650c8/ijerph-20-01865-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d223/9914633/283513d776b1/ijerph-20-01865-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d223/9914633/d1686c47012d/ijerph-20-01865-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d223/9914633/e6c127d3e28f/ijerph-20-01865-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d223/9914633/184377c80718/ijerph-20-01865-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d223/9914633/27d6e8f43901/ijerph-20-01865-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d223/9914633/889f5f3a6ce8/ijerph-20-01865-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d223/9914633/7731566650c8/ijerph-20-01865-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d223/9914633/283513d776b1/ijerph-20-01865-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d223/9914633/d1686c47012d/ijerph-20-01865-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d223/9914633/e6c127d3e28f/ijerph-20-01865-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d223/9914633/184377c80718/ijerph-20-01865-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d223/9914633/27d6e8f43901/ijerph-20-01865-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d223/9914633/889f5f3a6ce8/ijerph-20-01865-g007.jpg

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