Gurkan S, Bradley R M
Department of Oral Biology, University of Michigan, School of Dentistry, Ann Arbor 48109.
Brain Res. 1987 Sep 1;419(1-2):287-93. doi: 10.1016/0006-8993(87)90595-6.
To learn the nature of the autonomic control of the serous lingual salivary glands of von Ebner, parasympathetic and sympathetic agonists were injected into rats, and the extent of depletion of the secretory granules in gland acini was measured. Injection of the beta-adrenergic agonist isoproterenol caused a concentration-dependent reduction of the secretory granule content of the acinar cells. Injection of the beta-adrenergic antagonist propranolol, combined with isoproterenol, blocked the reduction in secretory granule content seen with isoproterenol alone. Injection of the parasympathetic agonist carbachol also produced a concentration-dependent reduction in granule content of the acini. This reduction was partially blocked by injection of atropine, and completely blocked by injection of atropine and propranolol. beta-Adrenergic and parasympathetic agonists alone did not cause total degranulation of the acini. However, this was achieved by injection of both agonists. It is concluded that protein secretion in von Ebner's glands is under both sympathetic and parasympathetic nervous control. It is hypothesized that the glands contain two sets of granules with different compositions, each under the control of either the parasympathetic or sympathetic nervous system. Because von Ebner's glands are closely associated with taste buds and because the glands supply the microenvironment of these taste buds the secretion of these glands may be very important in the mechanism of taste transduction.
为了解埃伯纳氏浆液性舌唾液腺的自主神经控制特性，将副交感神经和交感神经激动剂注射到大鼠体内，并测量腺泡中分泌颗粒的消耗程度。注射β-肾上腺素能激动剂异丙肾上腺素会导致腺泡细胞分泌颗粒含量呈浓度依赖性降低。注射β-肾上腺素能拮抗剂普萘洛尔并联合异丙肾上腺素，可阻断单独使用异丙肾上腺素时出现的分泌颗粒含量降低。注射副交感神经激动剂卡巴胆碱也会使腺泡颗粒含量呈浓度依赖性降低。注射阿托品可部分阻断这种降低，而注射阿托品和普萘洛尔则可完全阻断。单独使用β-肾上腺素能激动剂和副交感神经激动剂不会导致腺泡完全脱颗粒。然而，同时注射这两种激动剂可实现完全脱颗粒。得出的结论是，埃伯纳氏腺的蛋白质分泌受交感神经和副交感神经的双重控制。据推测，这些腺体含有两组成分不同的颗粒，每组颗粒分别受副交感神经系统或交感神经系统的控制。由于埃伯纳氏腺与味蕾密切相关，且这些腺体为味蕾提供微环境，因此这些腺体的分泌在味觉转导机制中可能非常重要。
