Department of Small Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Int J Mol Sci. 2023 Jan 18;24(3):1867. doi: 10.3390/ijms24031867.
Progress in prognostic factors, treatments, and outcome for both canine and human osteosarcoma (OS) has been minimal over the last three decades. Surface overexpression of the cation independent mannose-6-phosphate/insulin-like growth factor receptor type 2 (IGF2R) has been proven to occur in human OS cells. Subsequently, radioimmunotherapy (RIT) targeting IGF2R has demonstrated promising preliminary results. The main aims of this study were to investigate the expression of IGF2R in spontaneously occurring canine OS cells using immunohistochemistry (IHC) on archived biopsy samples and to assess its prognostic significance. Thirty-four dogs were included in the study. All cases showed that 80-100% of OS cells stained positive for IGF2R. IGF2R overexpression alone was not shown to have prognostic significance using both visual and quantitative methods of IHC staining intensity. This study has established for the first time the consistent expression of IGF2R in spontaneously occurring canine OS. This comparative oncology approach will allow further investigation into RIT as a novel treatment modality; first in canines and then in humans with OS. In addition, further studies should be performed to assess the true prognostic significance of IGF2R overexpression.
在过去的三十年中，犬和人骨肉瘤（OS）的预后因素、治疗方法和结果的进展甚微。已经证明，人骨肉瘤细胞表面过表达阳离子非依赖性甘露糖-6-磷酸/胰岛素样生长因子受体 2（IGF2R）。随后，针对 IGF2R 的放射免疫治疗（RIT）显示出了有希望的初步结果。本研究的主要目的是使用存档活检样本中的免疫组织化学（IHC）来检测 IGF2R 在自发性发生的犬骨肉瘤细胞中的表达，并评估其预后意义。本研究纳入了 34 只狗。所有病例均显示，80-100%的骨肉瘤细胞对 IGF2R 染色呈阳性。使用 IHC 染色强度的视觉和定量方法均未显示 IGF2R 过表达具有预后意义。本研究首次在自发性发生的犬骨肉瘤中建立了 IGF2R 的一致表达。这种比较肿瘤学方法将允许进一步研究 RIT 作为一种新的治疗方式；首先在犬中，然后在患有骨肉瘤的人中进行。此外，应进行进一步的研究来评估 IGF2R 过表达的真正预后意义。
