Schreyer D J, Jones E G
Department of Anatomy and Neurobiology, University of California, Irvine 92717.
Brain Res. 1987 Oct;432(2):291-9. doi: 10.1016/0165-3806(87)90054-x.
Nitrocellulose implants treated with biological materials known to support neurite growth in vitro were introduced at thoracolumbar levels of the neonatal rat spinal cord before the arrival of growing corticospinal tract (CST) axons. Implant placement was designed to interrupt the normal CST growth path and provide a potential, alternative growth path. Subsequent growth of CST axons within the spinal cord in the vicinity of the implants was evaluated by labeling the axons with the anterograde transport of horseradish peroxidase. Untreated implants either blocked further CST axon growth or deflected CST axons to abnormal positions. Implants bearing living cells from spinal cord primary cultures were able to support the adhesion and growth of CST axons. Similarly, acellular implants coated with laminin, but not with poly-L-lysine supported the adhesion and growth of CST axons, suggesting that laminin or some other adhesive factor produced by immature neuroglial cells may be normally involved in CST axon growth and guidance.
在新生大鼠脊髓的胸腰段，于生长中的皮质脊髓束（CST）轴突到达之前，植入经已知能在体外支持神经突生长的生物材料处理过的硝酸纤维素。植入物的放置旨在中断CST的正常生长路径，并提供一条潜在的替代生长路径。通过用辣根过氧化物酶的顺行运输标记轴突，评估植入物附近脊髓内CST轴突的后续生长情况。未处理的植入物要么阻止CST轴突进一步生长，要么将CST轴突偏转到异常位置。带有脊髓原代培养活细胞的植入物能够支持CST轴突的黏附和生长。同样，涂有层粘连蛋白而非聚-L-赖氨酸的无细胞植入物支持CST轴突的黏附和生长，这表明未成熟神经胶质细胞产生的层粘连蛋白或其他一些黏附因子可能正常参与CST轴突的生长和导向。
