Department of Obstetrics and Gynecology, Key Laboratory of Birth Defects and Related Diseases of Women and Children of MOE, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
Deep Underground Space Medical Center, West China Hospital, Sichuan University, Chengdu 610041, China.
Int J Mol Sci. 2023 Jan 31;24(3):2704. doi: 10.3390/ijms24032704.
Pelvic organ prolapse (POP) seriously affects elderly patients' quality of life, and new repair materials are urgently needed. To solve this problem, we synthesized methacrylated gelatin (GelMA) hydrogels and incorporated photothermally active Prussian blue nanoparticles (PBNPs) to synthesize PBNP@GelMA. Then, MSCs were encapsulated in the PBNP@GelMA and exposed to a 1.0 W/cm of 808 nm laser for 10 min to perform heat shock pretreatment for the implantation of mesenchymal stem cells (MSCs). Next, we tested the repair efficacy of scaffold-cell complexes both in vitro and in vivo. Our results reveal that the heat shock treatment induced by PBNP@GelMA improved the viability of MSCs, and the underlying mechanism may be related to HSP70. Furthermore, 2 weeks after implantation in the SD rat model, the collagen content increased in the MSC implantation group and PBNP@GelMA implantation group. However, the muscle regeneration at the implanting position was mostly enhanced after the implantation of the heat-shock-pretreated MSCs, which illustrates that heat shock treatment can further promote the MSC-mediated muscle regeneration. Therefore, manipulating the cell environment and providing proper heat stimulus by using PBNP@GelMA with NIR is a novel strategy to enhance the regenerative potential of MSCs and to promote pelvic tissue repair.
盆腔器官脱垂（POP）严重影响老年患者的生活质量，因此急需新型修复材料。为了解决这个问题，我们合成了甲基丙烯酰化明胶（GelMA）水凝胶，并将光热活性普鲁士蓝纳米颗粒（PBNPs）掺入其中以合成 PBNP@GelMA。然后，将 MSC 包封在 PBNP@GelMA 中，并在 1.0 W/cm 的 808nm 激光下照射 10min 以进行 MSC 植入的热休克预处理。接下来，我们在体外和体内测试了支架细胞复合物的修复效果。我们的结果表明，PBNP@GelMA 诱导的热休克处理提高了 MSC 的活力，其潜在机制可能与 HSP70 有关。此外，在 SD 大鼠模型中植入 2 周后，MSC 植入组和 PBNP@GelMA 植入组的胶原含量增加。然而，在植入经过热休克预处理的 MSC 后，植入部位的肌肉再生得到了较大增强，这表明热休克处理可以进一步促进 MSC 介导的肌肉再生。因此，通过使用具有 NIR 的 PBNP@GelMA 来操纵细胞环境并提供适当的热刺激是增强 MSC 再生潜力和促进盆腔组织修复的一种新策略。
